Invitrogen

TEM5 Polyclonal Antibody

Name: TEM5 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-20442
Availability: InStock

高级验证

3个已发表图片

2 篇参考文献

查看其他10个TEM5抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

TEM5 Antibody in Immunohistochemistry (IHC)

图: 1 / 7

TEM5 Antibody (PA5-20442) in IHC

Immunohistochemistry of TEM5 in human bladder tissue with TEM5 Polyclonal Antibody (Product # PA5-20442) at 5 µg/mL.

TEM5 Antibody in Immunocytochemistry (ICC/IF)

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

TEM5 Antibody — Figure 4 GPR124-dependent signaling controls pericyte migration. ( A ) Diagram of the knockdown system. ( B ) Immunoblot analysis of GPR124 protein level in control HBVPs and in HBVPs transduced with a lentivirus expressing an shRNA to silence endogenous GPR124. ( C ) Quantitative analyses of the results as shown in ( B ) and presented in the bar graph as the densitometry ratio of the control from four independent experiments. ( D ) Diagram of the overexpression system. ( E ) Immunoblot analysis of GPR124 proteins in HBVPs transfected with either control or gene construct encoding GPR124 (FLAG-GPR124, MOI = 50 and 100) to confirm overexpression of GPR124. ( F ) Quantitative analyses of the results as shown in ( E ) and presented in the bar graph as the densitometry ratio of the control from four independent experiments. ( G ) Cell viability was measured by the CCK8 assay in Ctrl-HBVPs and shGPR124-HBVP (n = 4). (H) Representative images of control HBVPs (control), shGPR124 HBVPs (shGPR124) and GPR124-overexpressing HBVPs (GPR124 OE) at the beginning (t = 0 h) and end (t = 24 h) of the wound healing assay. The dashed lines (black) indicate the edges of the wound immediately after scratch. The dashed lines (red) outline the area of cell migration. (I) Quantification of relative migration in a wound healing assay after 24 h (normalized to control values; n = 4 in each group). (J) Schematic representation of the microfluidic migration chamber used for observing the chemotactic re {KD}

产品信息

PA5-20442

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-4 µg/mL

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免疫组化 (IHC)

20 µg/mL

免疫组化（石蜡） (IHC (P))

5 µg/mL

免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human, Rat

已发表种属

Mouse

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A 14 amino acid synthetic peptide near the center of human TEM5. The immunogen is located within amino acids 560-610 of TEM5.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11152277

产品详细信息

A suggested positive control is rat kidney tissue lysate.

PA5-20442 can be used with blocking peptide PEP-0559.

Predicted species reactivity based on immunogen sequence: Mouse: (85%).

靶标信息

Tumor endothelial markers (TEMs) are significantly up-regulated during angiogenesis and neoangiogensis that are crucial for the growth of solid tumors. TEMs localized on the cell surface and conserved across species are of particular interest for future development of anti-angiogenic therapies. These include TEMs such as TEM1, TEM5, TEM7 and TEM8. TEM5 is a member of the adhesion family of G protein coupled receptors and is localized on the surface of endothelial cells. TEM5 is a seven-pass transmembrane receptor, unlike TEM1, TEM7 and TEM8 which span the membrane once. TEM5 is abundantly expressed in tumor vessels, heart, placenta, ovary, small intestine, and colon. Proteolytically processed soluble TEM5 mediates endothelial cell survival during angiogenesis by linking integrin to glycosaminoglycans.

仅用于科研。不用于诊断过程。未经明确授权不得转售。