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Agrisera
TGA1/TGACG motif-binding factor 1, bZIP transcription factor Polyclonal Antibody
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FIGURE: 1 / 1
TGA1/TGACG motif-binding factor 1, bZIP transcription factor Antibody (AS16-3208) in WB
50 µg of total protein from Arabidopsis thaliana seedlings of col-0, tga 1-1 and Nicotiana tabacum leaf transiently expressing TGA 1-GFP (described sample order from left to right), extracted with exaction buffer (50 m M Tris-HCl, p H 7.5,150 m M Na Cl, 5 m M EDTA, 0.1% Triton X-100,0.2% Nonidet P-4) and denatured at 70°C for 15 min were separated on 4-12% SDS-PAGE and blotted 1 h to nitrocellulose membrane... View More
Product Details
AS16-3208
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Plant
Host/Isotype
Rabbit
Class
Polyclonal
Type
Antibody
Immunogen
KLH-conjugated peptide derived from protein sequence of Arabidopsis thaliana TGA1.
Conjugate
Form
Lyophilized
Amount
50 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS, pH 7.4
Contains
no preservative
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
Product Specific Information
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
Target Information
TGA1 encodes a nuclear, sequence-specific DNA-binding transcription factor of the basic region/leucine zipper (bZIP) family that functions as a dimer and is enriched for an N-terminal regulatory region coupled to a C-terminal basic DNA-binding region followed by a leucine-zipper dimerization interface; consistent with bZIP proteins, the basic region confers recognition of ACGT-containing promoter elements, and TGA1 displays strong preference for TGACG-centered motifs (including ocs/as-1-type elements) that occur in defense- and hormone-responsive promoters. In Arabidopsis, TGA1 participates in transcriptional reprogramming during salicylic acid-associated immunity and systemic acquired resistance by binding TGACG/as-1-like cis elements and cooperating with the master cofactor NPR1, with NPR1 recruitment and/or activity linked to the redox state of conserved cysteine residues in TGA1 that modulate NPR1 interaction and downstream promoter activation.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: AT5G65210; AtTGA1; Q39237; TGA1; TGACG motif-binding factor 1; TGACG sequence-specific binding protein 1
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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