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          • Primary Antibodies ›
          • TGF beta-1,2,3 Antibodies

          Invitrogen

          TGF beta-1,2,3 Monoclonal Antibody (eBioTB2F), eBioscience™

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          Cite TGF beta-1,2,3 Monoclonal Antibody (eBioTB2F), eBioscience™

          Additional Information:
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          • 抗体检测数据 (1)
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          TGF beta-1,2,3 Antibody in ELISA (ELISA)
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          TGF beta-1,2,3 Antibody in ELISA (ELISA)
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          TGF beta-1,2,3 Antibody (14-9943-81) in ELISA

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          TGF beta-1,2,3 Antibody in ELISA (ELISA)
          TGF beta-1,2,3 Antibody in ELISA (ELISA)

          产品信息

          14-9943-81

          应用
          建议稀释比
          已发表文章

          酶联免疫吸附实验 (ELISA)

          6-10 µg/mL
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Human

          已发表种属

          Mouse

          宿主/亚型

          Rat / IgG2a, kappa

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          eBioTB2F

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.5 mg/mL

          规格

          50 µg

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2

          内含物

          0.09% sodium azide

          保存条件

          4°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1271967

          产品详细信息

          Description: The eBioTB2F antibody reacts with Transforming Growth Factor-beta (TGF-beta), a pleiotropic cytokine, exists in five isoforms, known as TGF-beta1-5. Homologies between isoforms range from 70-80% but no homology exists to TGF-alpha. TGF-beta1 is ubiquitous and the most abundant form found in lymphoid organs, while other isoforms are expressed in a more restricted distribution. The biologically active state of all isoforms are disulfide-linked homodimers. The heat- and acid- stable monomeric subunits have a length of 112 amino acids. The isoforms of TGF-beta arise by proteolytic cleavage of longer precursors; the isoforms are derived from the carboxyterminal ends of these precursors. Isoforms isolated from different species are evolutionarily closely conserved and have sequence identities on the order of 98%. Mature human, porcine, simian, chicken and bovine TGF-beta1 are identical and differ from mouse TGF-beta1 in a single amino acid. TGF-beta1 is produced in very high levels by platelets. Other cellular sources of TGF-beta1 include macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. TGF-beta1 secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. Activities of TGF-beta1 include inhibition of cell growth for inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neurons, and lymphoid cells and other hematopoietic cell types. TGF-beta1 inhibits the proliferation of T cells and NK cells and down regulates the activities of activated macrophages. TGF-beta1 blocks the anti-tumor activity of IL-2 - bearing lymphokine-activated killer (LAK) cells.

          Recently, TGF-beta1 has been found to have a critical role in the development of regulatory T cells. Dendritic cells exposed to tumors have been reported to secrete TGF-beta1 and stimulate expansion of natural T reg cells. Moreover, TGF-beta1 has been shown to act as a costimulatory factor for expression of Foxp3, leading to the differentiation of CD4+CD25+ Treg cells from peripheral CD4+CD25- progeny. TGF-beta-induced regulatory T cells have been termed Ti-Treg.

          Applications Reported: This eBioTB2F antibody has been reported for use in ELISA.

          Applications Tested: The eBioTB2F antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human and mouse TGF-beta in combination with the detection eBio16TFB (Product # 13-9923-81) antibody for detection. A suitable range of concentrations of this antibody for ELISA capture is 6-10 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 8000 pg/mL - 60 pg/mL should be included in each ELISA plate.

          This antibody recognizes the mature/active form of TGF beta 1 without association with Latency Associated Peptide (LAP). Most samples will require acid-treatment and neutralization to remove LAP from TGF beta 1 prior to evaluation in this assay. Samples should be tested in the assay immediately after acid treatment and neutralization. It is also possible that some serum and plasma samples may contain low levels of immunoreactive TGF beta 1 that has disassociated from LAP. Naturally occurring, free TGF beta 1 may be measurable by evaluating samples without acid treatment.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          靶标信息

          TGF-beta1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta1, -2, and -3 are disulfide-linked homodimers.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: BSC-1 cell growth inhibitor; Cetermin; G-TSF; Glioblastoma-derived T-cell suppressor factor; latency-associated peptide; polyergin; prepro-transforming growth factor beta-1; prepro-transforming growth factor beta-2; prepro-transforming growth factor beta-3; tgf beta 1; tgf beta 3; tgf beta1; TGF-beta-1; Transforming growth factor; Transforming growth factor beta-1 proprotein; Transforming growth factor beta-2 proprotein; Transforming growth factor beta-3 proprotein; transforming growth factor beta1; transforming growth factor-beta-2 precursor; transforming growth factor-beta3; unnamed protein product

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          基因别名: ARVD; ARVD1; CED; DPD1; G-TSF; IBDIMDE; LAP; LDS4; LDS5; RNHF; TGF-beta1; TGF-beta2; TGF-beta3; TGFB; TGFB1; TGFB2; TGFB3; TGFbeta

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          UniProt ID: (Human) P01137, (Human) P61812, (Human) P10600

          查看更多 收起

          Entrez Gene ID: (Human) 7040, (Human) 7042, (Human) 7043

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          功能
          type II transforming growth factor beta receptor binding cytokine activity transforming growth factor beta receptor binding protein binding growth factor activity enzyme binding type I transforming growth factor beta receptor binding type III transforming growth factor beta receptor binding deubiquitinase activator activity identical protein binding protein serine/threonine kinase activator activity macromolecular complex binding beta-amyloid binding receptor binding protein homodimerization activity transforming growth factor beta binding
          参与通路
          negative regulation of transcription from RNA polymerase II promoter cell morphogenesis vasculogenesis ureteric bud development response to hypoxia morphogenesis of a branching structure cell activation epithelial to mesenchymal transition neural tube closure regulation of sodium ion transport sprouting angiogenesis chondrocyte differentiation columnar/cuboidal epithelial cell maturation hematopoietic progenitor cell differentiation connective tissue replacement involved in inflammatory response wound healing adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains tolerance induction to self antigen negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target heart valve morphogenesis aortic valve morphogenesis protein export from nucleus ATP biosynthetic process phosphate-containing compound metabolic process cellular calcium ion homeostasis apoptotic process inflammatory response transforming growth factor beta receptor signaling pathway Notch signaling pathway negative regulation of neuroblast proliferation salivary gland morphogenesis endoderm development heart development female pregnancy cell proliferation positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration response to xenobiotic stimulus response to wounding response to glucose gene expression regulation of gene expression positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of extracellular matrix disassembly positive regulation of epithelial to mesenchymal transition macrophage derived foam cell differentiation positive regulation of fibroblast migration negative regulation of macrophage cytokine production oligodendrocyte development positive regulation of microglia differentiation regulation of striated muscle tissue development neural tube development hyaluronan catabolic process T cell differentiation negative regulation of ossification negative regulation of cell growth osteoclast differentiation regulation of cell migration positive regulation of cell migration mammary gland development organ regeneration membrane protein intracellular domain proteolysis positive regulation of protein complex assembly positive regulation of exit from mitosis response to estradiol response to progesterone regulation of interleukin-23 production negative regulation of interleukin-17 production positive regulation of interleukin-17 production positive regulation of interleukin-6 production positive regulation of tumor necrosis factor production receptor catabolic process positive regulation of superoxide anion generation mononuclear cell proliferation regulation of actin cytoskeleton organization positive regulation of collagen biosynthetic process response to vitamin D response to laminar fluid shear stress response to immobilization stress myofibroblast differentiation T cell proliferation T cell activation regulation of cell proliferation negative regulation of T cell proliferation regulation of protein import into nucleus positive regulation of protein import into nucleus odontogenesis of dentin-containing tooth positive regulation of odontogenesis myelination T cell homeostasis positive regulation of apoptotic process positive regulation of vascular permeability positive regulation of I-kappaB kinase/NF-kappaB signaling surfactant homeostasis proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of MAPK cascade positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration regulatory T cell differentiation cell-cell junction organization response to ethanol regulation of regulatory T cell differentiation positive regulation of regulatory T cell differentiation negative regulation of cell differentiation positive regulation of cell differentiation negative regulation of fat cell differentiation negative regulation of myoblast differentiation positive regulation of epidermal growth factor receptor signaling pathway negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter muscle cell cellular homeostasis positive regulation of fibroblast proliferation lung alveolus development positive regulation of isotype switching to IgA isotypes lymph node development digestive tract development negative regulation of skeletal muscle tissue development positive regulation of smooth muscle cell proliferation inner ear development epithelial cell proliferation positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation positive regulation of protein secretion positive regulation of inflammatory response negative regulation of phagocytosis defense response to fungus negative regulation of T cell activation positive regulation of chemotaxis positive regulation of cellular component organization positive regulation of smooth muscle cell differentiation positive regulation of multicellular organismal process positive regulation of protein metabolic process negative regulation of release of sequestered calcium ion into cytosol neuron apoptotic process regulation of cell cycle positive regulation of cell division positive regulation of protein kinase B signaling ventricular cardiac muscle tissue morphogenesis phospholipid homeostasis canonical Wnt signaling pathway regulation of blood vessel remodeling face morphogenesis frontal suture morphogenesis positive regulation of SMAD protein import into nucleus bronchiole development mammary gland branching involved in thelarche branch elongation involved in mammary gland duct branching regulation of branching involved in mammary gland duct morphogenesis regulation of cartilage development retina vasculature development in camera-type eye connective tissue development Langerhans cell differentiation negative regulation of biomineral tissue development regulation of enamel mineralization lens fiber cell differentiation positive regulation of ERK1 and ERK2 cascade response to cholesterol cellular response to mechanical stimulus cellular response to glucose stimulus cellular response to growth factor stimulus cellular response to low-density lipoprotein particle stimulus cellular response to hypoxia cellular response to ionizing radiation cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus positive regulation of mononuclear cell migration odontoblast differentiation stem cell proliferation extracellular matrix assembly positive regulation of branching involved in ureteric bud morphogenesis positive regulation of canonical Wnt signaling pathway extrinsic apoptotic signaling pathway liver regeneration cellular response to virus transforming growth factor beta receptor superfamily signaling pathway negative regulation of hyaluronan biosynthetic process positive regulation of protein localization to nucleus positive regulation of extracellular matrix assembly response to salt positive regulation of mesenchymal stem cell proliferation regulation of miRNA transcription negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of protein localization to plasma membrane positive regulation of vasculature development positive regulation of STAT cascade regulation of epithelial to mesenchymal transition involved in endocardial cushion formation cellular response to acetaldehyde cellular response to insulin-like growth factor stimulus embryonic liver development negative regulation of cell-cell adhesion mediated by cadherin positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of endothelial cell apoptotic process positive regulation of primary miRNA processing positive regulation of stem cell proliferation positive regulation of cardiac muscle cell differentiation in utero embryonic development odontogenesis uterine wall breakdown negative regulation of neuron apoptotic process positive regulation of filopodium assembly positive regulation of stress fiber assembly secondary palate development detection of hypoxia negative regulation of vascular smooth muscle cell proliferation positive regulation of occluding junction disassembly skeletal system development cartilage condensation blood vessel development eye development kidney development hair follicle development blood vessel remodeling heart morphogenesis outflow tract septum morphogenesis membranous septum morphogenesis outflow tract morphogenesis atrioventricular valve morphogenesis pulmonary valve morphogenesis endocardial cushion morphogenesis cardiac right ventricle morphogenesis ventricular trabecula myocardium morphogenesis endocardial cushion fusion atrial septum primum morphogenesis neural retina development activation-induced cell death of T cells axon guidance sensory organ development glial cell migration male gonad development embryo development ending in birth or egg hatching cardioblast differentiation positive regulation of epithelial cell migration striated muscle tissue development cell migration negative regulation of angiogenesis signaling hemopoiesis extracellular matrix organization collagen fibril organization positive regulation of cell growth embryonic limb morphogenesis neutrophil chemotaxis epithelial cell differentiation hair follicle morphogenesis positive regulation of stress-activated MAPK cascade regulation of transforming growth factor beta2 production positive regulation of cell adhesion mediated by integrin ascending aorta morphogenesis wound healing dopamine biosynthetic process regulation of apoptotic process positive regulation of neuron apoptotic process positive regulation of integrin biosynthetic process positive regulation of Notch signaling pathway positive regulation of cell cycle positive regulation of heart contraction negative regulation of Ras protein signal transduction somatic stem cell division embryonic digestive tract development neuron fate commitment neuron development generation of neurons positive regulation of immune response regulation of timing of catagen positive regulation of catagen positive regulation of cardioblast differentiation cardiac muscle cell proliferation uterus development cardiac epithelial to mesenchymal transition ventricular septum morphogenesis atrial septum morphogenesis negative regulation of cartilage development pharyngeal arch artery morphogenesis positive regulation of cardiac epithelial to mesenchymal transition positive regulation of activation-induced cell death of T cells positive regulation of extracellular matrix disassembly extrinsic apoptotic signaling pathway in absence of ligand regulation of apoptotic process involved in outflow tract morphogenesis regulation of extracellular matrix organization regulation of complement-dependent cytotoxicity substantia propria of cornea development pericyte cell differentiation cranial skeletal system development negative regulation of epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of extrinsic apoptotic signaling pathway in absence of ligand
          It has to be done as per old AB suggested Products section.

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