Hamburger Menu Button
Thermo Fisher Scientific Logo
登录
没有账号?建立账号 注册
  • 产品
    • 抗体
    • 引物合成
    • GeneArt Gene Synthesis Instant Designer
    • TaqMan Assay and Array Search
    • PCR 设备和耗材
    • 色谱柱和纯化柱
    • 实验室离心机
    • 分子生物学试剂和试剂盒
    • 实验室塑料耗材及用品
    • 查看所有产品分类
  • 应用和技术
    • 色谱
    • 细胞转染和培养
    • 实时荧光定量PCR
    • 质谱
    • 实验室塑料耗材及用品
    • 电子显微镜
    • 蛋白质生物学
    • PCR
    • RNAi
    • 环境
    • 查看所有应用和技术
  • 服务
    • 定制服务
    • 企业级实验室信息化服务
    • 企业服务
    • 金融&租赁服务
    • 仪器服务
    • OEM和商业供应
    • 培训服务
    • 360° CDMO 和 CRO 服务
    • CDMO 服务
    • 临床试验 CRO 服务
    • 查看所有帮助和支持
  • 帮助和支持
    • 学习中心
    • 联系我们
    • 更改国家或地区
    • 查看所有帮助和支持
  • 热门产品
    • TaqMan Real-Time PCR Assays
      TaqMan 实时荧光定量 PCR 试剂盒
    • Antibodies
      抗体
    • Oligos, Primers & Probes
      寡核苷酸、引物、探针
    • GeneArt Gene Synthesis
      GeneArt 基因合成
    • Cell Culture Plastics
      细胞培养耗材
  • 联系我们
  • 货号直购
  • 订单查询
  • 查找文件&证书
Thermo Fisher Scientific Logo

Search

搜索全部或下列指定分类
Search button
          • 联系我们
          • 货号直购
          • 登录
            登录
            没有账号?建立账号 注册
            • 我的账号
            • 订单
            • 账单及发票查询
            • 定制产品及项目

          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TGF beta-1,2,3 Antibodies

          Invitrogen

          TGF beta-1,2,3 Monoclonal Antibody (eBioTB2F), eBioscience™

          1个已发表图片
          1 篇参考文献
          查看其他9个TGF beta-1,2,3抗体

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promotions']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.viewpromo']}}

          {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.promocode']}}: {{promo.promoCode}} {{promo.promoTitle}} {{promo.promoDescription}}. {{$productOrderCtrl.translations['antibody.pdp.commerceCard.promotion.learnmore']}}

          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite TGF beta-1,2,3 Monoclonal Antibody (eBioTB2F), eBioscience™

          • 抗体检测数据 (1)
          • 已发表图片 (1)
          TGF beta-1,2,3 Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.
          TGF beta-1,2,3 Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.

          图: 1 / 2

          {{ $ctrl.currentElement.advancedVerification.fullName }}
          {{ $ctrl.currentElement.advancedVerification.text }}

          TGF beta-1,2,3 Antibody (14-9943-81) in ELISA

          Standard curve of Anti-Human TGF beta ELISA. Recombinant cytokine standard concentration in pg/mL. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

          {{$ctrl.videoDesc}}

          {{$ctrl.videoLongDesc}}

          TGF beta-1,2,3 Antibody in ELISA (ELISA)
          TGF beta-1,2,3 Antibody in ELISA (ELISA)

          产品信息

          14-9943-81

          应用
          建议稀释比
          已发表文章

          酶联免疫吸附实验 (ELISA)

          6-10 µg/mL
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Human

          已发表种属

          Mouse

          宿主/亚型

          Rat / IgG2a, kappa

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          eBioTB2F

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.5 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2

          内含物

          0.09% sodium azide

          保存条件

          4°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_1271967

          产品详细信息

          Description: The eBioTB2F antibody reacts with Transforming Growth Factor-beta (TGF-beta), a pleiotropic cytokine, exists in five isoforms, known as TGF-beta1-5. Homologies between isoforms range from 70-80% but no homology exists to TGF-alpha. TGF-beta1 is ubiquitous and the most abundant form found in lymphoid organs, while other isoforms are expressed in a more restricted distribution. The biologically active state of all isoforms are disulfide-linked homodimers. The heat- and acid- stable monomeric subunits have a length of 112 amino acids. The isoforms of TGF-beta arise by proteolytic cleavage of longer precursors; the isoforms are derived from the carboxyterminal ends of these precursors. Isoforms isolated from different species are evolutionarily closely conserved and have sequence identities on the order of 98%. Mature human, porcine, simian, chicken and bovine TGF-beta1 are identical and differ from mouse TGF-beta1 in a single amino acid. TGF-beta1 is produced in very high levels by platelets. Other cellular sources of TGF-beta1 include macrophages, lymphocytes, endothelial cells, chondrocytes, and leukemic cells. TGF-beta1 secretion can be induced by steroids, retinoids, EGF, NGF, vitamin D3, and IL-1. Activities of TGF-beta1 include inhibition of cell growth for inhibitor for normal and transformed epithelial cells, endothelial cells, fibroblasts, neurons, and lymphoid cells and other hematopoietic cell types. TGF-beta1 inhibits the proliferation of T cells and NK cells and down regulates the activities of activated macrophages. TGF-beta1 blocks the anti-tumor activity of IL-2 - bearing lymphokine-activated killer (LAK) cells.

          Recently, TGF-beta1 has been found to have a critical role in the development of regulatory T cells. Dendritic cells exposed to tumors have been reported to secrete TGF-beta1 and stimulate expansion of natural T reg cells. Moreover, TGF-beta1 has been shown to act as a costimulatory factor for expression of Foxp3, leading to the differentiation of CD4+CD25+ Treg cells from peripheral CD4+CD25- progeny. TGF-beta-induced regulatory T cells have been termed Ti-Treg.

          Applications Reported: This eBioTB2F antibody has been reported for use in ELISA.

          Applications Tested: The eBioTB2F antibody has been tested as the capture antibody in a sandwich ELISA for analysis of human and mouse TGF-beta in combination with the detection eBio16TFB (Product # 13-9923-81) antibody for detection. A suitable range of concentrations of this antibody for ELISA capture is 6-10 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 8000 pg/mL - 60 pg/mL should be included in each ELISA plate.

          This antibody recognizes the mature/active form of TGF beta 1 without association with Latency Associated Peptide (LAP). Most samples will require acid-treatment and neutralization to remove LAP from TGF beta 1 prior to evaluation in this assay. Samples should be tested in the assay immediately after acid treatment and neutralization. It is also possible that some serum and plasma samples may contain low levels of immunoreactive TGF beta 1 that has disassociated from LAP. Naturally occurring, free TGF beta 1 may be measurable by evaluating samples without acid treatment.

          Purity: Greater than 90%, as determined by SDS-PAGE.

          Aggregation: Less than 10%, as determined by HPLC.

          Filtration: 0.2 µm post-manufacturing filtered.

          靶标信息

          TGF-beta1, -2, and -3 are a closely related group of proteins (70-80% sequence homology) that are produced by many cell types and function as growth and differentiation factors. The active forms of TGF-beta1, -2, and -3 are disulfide-linked homodimers.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: BSC-1 cell growth inhibitor; Cetermin; G-TSF; Glioblastoma-derived T-cell suppressor factor; latency-associated peptide; polyergin; prepro-transforming growth factor beta-1; prepro-transforming growth factor beta-2; prepro-transforming growth factor beta-3; tgf beta 1; tgf beta 3; tgf beta1; TGF-beta-1; TGF-beta-3; Transforming growth factor; Transforming growth factor beta-1 proprotein; Transforming growth factor beta-2 proprotein; Transforming growth factor beta-3 proprotein; transforming growth factor, beta 2; transforming growth factor, beta 3

          查看更多 收起

          基因别名: ARVD; ARVD1; CED; DPD1; LAP; LDS4; LDS5; RNHF; TGF-beta2; TGF-beta3; TGFB; TGFB1; TGFB2; TGFB3; TGFbeta

          查看更多 收起

          UniProt ID: (Human) P01137, (Human) P61812, (Human) P10600

          查看更多 收起

          Entrez Gene ID: (Human) 7040, (Human) 7042, (Human) 7043

          查看更多 收起

          功能
          glycoprotein binding antigen binding type II transforming growth factor beta receptor binding cytokine activity transforming growth factor beta receptor binding protein binding growth factor activity enzyme binding type I transforming growth factor beta receptor binding type III transforming growth factor beta receptor binding protein homodimerization activity protein serine/threonine kinase activator activity protein heterodimerization activity protein N-terminus binding beta-amyloid binding receptor signaling protein serine/threonine kinase activity receptor binding identical protein binding transforming growth factor beta binding growth factor
          参与通路
          protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade ureteric bud development response to hypoxia epithelial to mesenchymal transition negative regulation of protein phosphorylation positive regulation of protein phosphorylation regulation of sodium ion transport chondrocyte differentiation hematopoietic progenitor cell differentiation connective tissue replacement involved in inflammatory response wound healing adaptive immune response based on somatic recombination of immune receptors built from immunoglobulin superfamily domains tolerance induction to self antigen platelet degranulation protein phosphorylation protein export from nucleus ATP biosynthetic process phosphate-containing compound metabolic process cellular calcium ion homeostasis inflammatory response cell cycle arrest mitotic cell cycle checkpoint epidermal growth factor receptor signaling pathway transforming growth factor beta receptor signaling pathway common-partner SMAD protein phosphorylation SMAD protein complex assembly SMAD protein import into nucleus Notch signaling pathway negative regulation of neuroblast proliferation salivary gland morphogenesis endoderm development female pregnancy aging negative regulation of DNA replication positive regulation of cell proliferation negative regulation of cell proliferation germ cell migration response to radiation response to wounding response to glucose defense response to fungus, incompatible interaction positive regulation of vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression negative regulation of extracellular matrix disassembly positive regulation of epithelial to mesenchymal transition macrophage derived foam cell differentiation positive regulation of fibroblast migration positive regulation of peptidyl-threonine phosphorylation positive regulation of pathway-restricted SMAD protein phosphorylation negative regulation of macrophage cytokine production oligodendrocyte development cell growth regulation of striated muscle tissue development cell migration regulation of transforming growth factor beta receptor signaling pathway evasion or tolerance of host defenses by virus negative regulation of cell-cell adhesion hyaluronan catabolic process negative regulation of ossification negative regulation of cell growth regulation of cell migration positive regulation of cell migration positive regulation of bone mineralization negative regulation of transforming growth factor beta receptor signaling pathway positive regulation of histone deacetylation organ regeneration positive regulation of protein complex assembly positive regulation of exit from mitosis lipopolysaccharide-mediated signaling pathway positive regulation of cellular protein metabolic process response to estradiol response to progesterone regulation of interleukin-23 production negative regulation of interleukin-17 production positive regulation of interleukin-17 production receptor catabolic process positive regulation of superoxide anion generation mononuclear cell proliferation positive regulation of collagen biosynthetic process positive regulation of peptidyl-serine phosphorylation response to vitamin D response to laminar fluid shear stress positive regulation of histone acetylation positive regulation of protein dephosphorylation negative regulation of T cell proliferation regulation of protein import into nucleus positive regulation of protein import into nucleus positive regulation of odontogenesis response to drug myelination regulation of apoptotic process myeloid dendritic cell differentiation T cell homeostasis positive regulation of apoptotic process positive regulation of vascular permeability positive regulation of MAP kinase activity protein kinase B signaling positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration positive regulation of phosphatidylinositol 3-kinase activity ossification involved in bone remodeling regulatory T cell differentiation cell-cell junction organization positive regulation of regulatory T cell differentiation negative regulation of cell differentiation negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of cell cycle negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter active induction of host immune response by virus positive regulation of fibroblast proliferation positive regulation of isotype switching to IgA isotypes lymph node development digestive tract development negative regulation of skeletal muscle tissue development inner ear development positive regulation of epithelial cell proliferation negative regulation of epithelial cell proliferation positive regulation of protein secretion positive regulation of peptidyl-tyrosine phosphorylation negative regulation of phagocytosis positive regulation of chemotaxis positive regulation of NF-kappaB transcription factor activity regulation of binding regulation of DNA binding positive regulation of smooth muscle cell differentiation negative regulation of release of sequestered calcium ion into cytosol positive regulation of cell division positive regulation of protein kinase B signaling regulation of blood vessel remodeling face morphogenesis frontal suture morphogenesis pathway-restricted SMAD protein phosphorylation regulation of SMAD protein import into nucleus positive regulation of SMAD protein import into nucleus SMAD protein signal transduction mammary gland branching involved in thelarche branch elongation involved in mammary gland duct branching regulation of branching involved in mammary gland duct morphogenesis negative regulation of gene silencing by miRNA regulation of cartilage development lens fiber cell differentiation response to cholesterol positive regulation of cell cycle arrest cellular response to organic cyclic compound cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus extracellular matrix assembly positive regulation of branching involved in ureteric bud morphogenesis extrinsic apoptotic signaling pathway negative regulation of hyaluronan biosynthetic process positive regulation of extracellular matrix assembly positive regulation of NAD+ ADP-ribosyltransferase activity positive regulation of pri-miRNA transcription from RNA polymerase II promoter negative regulation of protein localization to plasma membrane negative regulation of production of miRNAs involved in gene silencing by miRNA positive regulation of receptor clustering regulation of epithelial to mesenchymal transition involved in endocardial cushion formation regulation of actin cytoskeleton reorganization positive regulation of transcription regulatory region DNA binding positive regulation of cardiac muscle cell differentiation cell morphogenesis cartilage condensation angiogenesis eye development hair follicle development blood vessel remodeling heart morphogenesis outflow tract septum morphogenesis membranous septum morphogenesis heart valve morphogenesis atrioventricular valve morphogenesis pulmonary valve morphogenesis endocardial cushion morphogenesis cardiac right ventricle morphogenesis ventricular trabecula myocardium morphogenesis endocardial cushion fusion atrial septum primum morphogenesis cell-cell signaling axon guidance heart development cell death cell proliferation glial cell migration embryo development cardioblast differentiation positive regulation of epithelial cell migration negative regulation of alkaline phosphatase activity positive regulation of phosphatidylinositol 3-kinase signaling signal transduction by protein phosphorylation hemopoiesis collagen fibril organization positive regulation of cell growth neutrophil chemotaxis hair follicle morphogenesis activation of protein kinase activity positive regulation of stress-activated MAPK cascade regulation of transforming growth factor beta2 production regulation of actin cytoskeleton organization positive regulation of cell adhesion mediated by integrin ascending aorta morphogenesis wound healing dopamine biosynthetic process odontogenesis catagen uterine wall breakdown positive regulation of neuron apoptotic process positive regulation of integrin biosynthetic process positive regulation of ossification positive regulation of cell cycle positive regulation of heart contraction negative regulation of Ras protein signal transduction somatic stem cell division embryonic digestive tract development neuron fate commitment neuron development generation of neurons negative regulation of immune response positive regulation of immune response positive regulation of catagen positive regulation of cardioblast differentiation cardiac muscle cell proliferation cardiac epithelial to mesenchymal transition ventricular septum morphogenesis atrial septum morphogenesis negative regulation of cartilage development pharyngeal arch artery morphogenesis positive regulation of activation-induced cell death of T cells positive regulation of extracellular matrix disassembly positive regulation of protein localization to nucleus regulation of apoptotic process involved in outflow tract morphogenesis regulation of complement-dependent cytotoxicity positive regulation of GTP binding negative regulation of epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of epithelial to mesenchymal transition involved in endocardial cushion formation positive regulation of extrinsic apoptotic signaling pathway in absence of ligand activation of MAPK activity in utero embryonic development mammary gland development regulation of cell proliferation regulation of MAPK cascade negative regulation of neuron apoptotic process response to estrogen positive regulation of DNA replication lung alveolus development embryonic neurocranium morphogenesis positive regulation of filopodium assembly positive regulation of stress fiber assembly palate development detection of hypoxia positive regulation of occluding junction disassembly
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

          了解更多
          help_icon

          随时为您服务

          获取常见问题的专家解答,或者直接联系我们的技术专家,咨询应用、设备、常规产品使用问题。

          联系技术支持
          订购 Plus Icon Minus Icon
          • 订单状态查询
          • 订单支持
          • 货号直购
          • 现货供应中心
          • 电子采购
          支持 Plus Icon Minus Icon
          • 帮助&支持
          • 联系我们 - 400 820 8982
          • 技术支持中心
          • 查找文件&证书
          • 报告网站问题
          资源 Plus Icon Minus Icon
          • 学习中心
          • 促销
          • 活动&研讨会
          • 社交媒体
          关于赛默飞 Plus Icon Minus Icon
          • 关于我们
          • 招聘 招聘
          • 投资者关系 投资者关系
          • 新闻 新闻
          • 社会责任 社会责任
          • 商标
          • 政策和通知
          品牌 Plus Icon Minus Icon
          • Thermo Scientific
          • Applied Biosystems
          • Invitrogen
          • Gibco
          • Ion Torrent
          • Patheon
          • PPD
          • 服务条款
          • 隐私政策
          • 定价和运输保险政策
          © 2006-2025 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific and its subsidiaries unless otherwise specified.
          China flag icon
          China
          本网站销售的所有产品均不得用于人类或动物之临床诊断或治疗,仅可用于工业或者科研等非医疗目的。
             电子营业执照 | 经营证照公示 | ICP备案号:沪ICP备11003924号 | 沪公网安备 31011502006935号

          Your items have has been added!


          Host server : magellan-search-blue-555ccb4fbd-tb6bv:80/100.66.78.132:80.
          git-commit: 75f0c7d4cb44686da2af39b21f2e6446a20282e2
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.41.0-2025.12.99-1.0