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Invitrogen
TGN46 Monoclonal Antibody (2F7.1)
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TGN46 Antibody (MA3-063) in ICC/IF
HeLa and COS7 cells were rinsed with PBS and fixed with -20°C methanol for 5 minutes. Cells were incubated with anti-TGN38 antibody (Product # MA3-063) diluted 1:100 in PBS containing 0.2%BSA for 1 hour. The cells were then rinsed in PBS and incubated with goat anti-mouse IgG conjugated to rhodamine for 30 minutes in PBS containing 0.2% BSA. Following washing, cells were imaged using a Zeiss Axioplan microscope. Data courtesy of the Innovators Program.
产品信息
MA3-063
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Non-human primate,
Rat
已发表种属
C. elegans,
Hamster,
Human,
Mouse,
Non-human primate,
Rat
宿主/亚型
Mouse
/ IgG1
分类
Monoclonal
类型
Antibody
克隆号
2F7.1
抗原
Synthetic peptide corresponding to residues L(18) P S A S K P N N T S S E N N P P(34) C of rat TGN38.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 1mg/mL BSA
内含物
0.05% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA3-063 detects trans-Golgi network (TGN) 38 from human and monkey samples and both recombinant and endogenous rat samples.
MA3-063 has been successfully used in Western blot, immunofluorescence, immunocytochemistry and FACS procedures. By Western blot, this antibody detects an ~38 kDa protein representing deglycosylated recombinant rat TGN38. Under normal Western blot conditions, this antibody detects an 85-95 kDa band depending on cell type. Immunofluorescence staining of TGN38 in NRK cells with MA3-063 results in staining predominantly in the TGN.
In immunofluorescence, it has been shown that methanol works best as a fixative.
The MA3-063 immunogen is a synthetic peptide corresponding to residues L(18) P S A S K P N N T S S E N N P P(34) C of rat TGN38.
靶标信息
IASPP is one of the most evolutionarily conserved inhibitors of p53 (TP53; MIM 191170), whereas ASPP1. and ASPP2. are activators of p53.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: hTGN48; hTGN51; TGN38 homolog; Trans-Golgi network glycoprotein 46; Trans-Golgi network glycoprotein 48; Trans-Golgi network glycoprotein 51; Trans-Golgi network integral membrane protein 2; Trans-Golgi network integral membrane protein TGN38; trans-Golgi network protein (46, 48, 51kD isoforms); trans-golgi network protein 1; Trans-Golgi network protein 2; trans-Golgi network protein TGN51
基因别名: TGN38; TGN46; TGN48; TGN51; Tgoln1; TGOLN2; Ttgn1; TTGN2
UniProt ID: (Human) O43493, (Rat) P19814
Entrez Gene ID: (Human) 10618, (Rat) 192152
