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Invitrogen
TIF1 gamma Polyclonal Antibody
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TIF1 gamma Antibody (PA5-80187) in ICC/IF
Immunocytochemistry/Immunofluorescence analysis of TIF1 gamma in A431 cells using TIF1 gamma Polyclonal Antibody (Product # PA5-80187). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 2 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibody at 1:100 ... View More
产品信息
PA5-80187
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human TIF1 gamma recombinant protein (Position: M1001-K1127).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 5mg BSA
内含物
0.05mg sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human K562 whole cell, human Jurkat whole cell, rat brain tissue, mouse brain tissue. IHC: human lung adenocarcinoma tissue, human ovarian serous adenocarcinoma tissue. ICC/IF: A431 cell. Flow: U87 cell, A431 cell.
靶标信息
TIF1 gamma is a member of the Transcriptional Intermediary Factor 1 family, which serve as coactivators and corepressors. Its structure is similar to TIF1 alpha and TIF1 beta, exhibiting multiple domains (RING finger, coiled coil, B boxes, PHD/TTC, and bromodomain). TIF1 gamma functions in cell differentiation and development, playing a role in differentiation of hematopoietic cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E3 ubiquitin-protein ligase TRIM33; Ectodermin homolog; Protein Rfg7; RET-fused gene 7 protein; RING-type E3 ubiquitin transferase TRIM33; TIF 1-g; TIF1-gamma; Transcription intermediary factor 1-gamma; transcriptional intermediary factor 1 gamma; tripartite motif protein 33; tripartite motif-containing 33; Tripartite motif-containing protein 33
基因别名: 8030451N04Rik; AI413936; ECTO; KIAA1113; PTC7; RFG7; TF1G; TIF1G; TIF1GAMMA; TIFGAMMA; TRIM33
UniProt ID: (Human) Q9UPN9, (Mouse) Q99PP7
Entrez Gene ID: (Human) 51592, (Rat) 365894, (Mouse) 94093
DNA binding
ubiquitin-protein transferase activity
protein binding
zinc ion binding
ligase activity
co-SMAD binding
R-SMAD binding
molecular_function
UDP-N-acetylmuramoylalanyl-D-glutamyl-2,6-diaminopimelate-D-alanyl-D-alanine ligase activity
ribosomal S6-glutamic acid ligase activity
coenzyme F420-0 gamma-glutamyl ligase activity
coenzyme F420-2 alpha-glutamyl ligase activity
metal ion binding
protein-glycine ligase activity
protein-glycine ligase activity, initiating
protein-glycine ligase activity, elongating
tubulin-glycine ligase activity
protein-glutamic acid ligase activity
tubulin-glutamic acid ligase activity
chromatin/chromatin-binding, or -regulatory protein
negative regulation of transcription from RNA polymerase II promoter
transcription, DNA-templated
protein ubiquitination
regulation of transforming growth factor beta receptor signaling pathway
negative regulation of BMP signaling pathway
negative regulation of transcription, DNA-templated
biological_process
regulation of transcription, DNA-templated
