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Invitrogen
TIM-3 Polyclonal Antibody
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FIGURE: 1 / 1
TIM-3 Antibody (PA5-79346) in WB
Western blot analysis of TIM-3 in Lane 1: human Raji whole cell lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was blocked with 5% Non-fat Milk/TBS for 1.5 hours at room temperature, incubated with TIM-3 polyclonal antibody (Product # PA5-79346) at a dilution of 0.5 µg/mL (overnight at 4°C), followed by goat anti-rabbit IgG-HRP secondary... View More
Product Details
PA5-79346
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
E. coli-derived human TIM 3 recombinant protein (Position: S22-G202).
Conjugate
Form
Lyophilized
Concentration
500 µg/mL
Amount
100 µg
Purification
Antigen affinity chromatography
Storage buffer
PBS with 4mg trehalose
Contains
0.05mg sodium azide
Storage conditions
-20°C
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Positive Control - WB: human Raji whole cell.
Target Information
TIM3 (Hepatitis A virus cellular receptor 2, HAVCR2, T-cell immunoglobulin, mucin-dmain containing-3) is a 281 amino acid long, Type-1 Th1- specific cell surface glycoprotein expressed on terminally differentiated CD4+Th1 and CD8+Tc1 cells. TIM3 consists of an IgV-like domain, a mucin-like domain in the extracellular region, and a conserved Tyrosine phosphorylation motif in the cytoplasmic region. TIM3 is involved in macrophage activation and induction of autoimmune diseases. Further, TIM3 down-regulates aggressive Th1-mediated immune responses and facilitates in the development of immune tolerance. Pathological significance of TIM3 has been attributed to Experimental autoimmune encephalomyelitis (EAE), a Th-1 dependent autoimmune disease, and also enhances the severity of experimental autoimmune encephalomyelitis in mice.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: CD366; FLJ14428; HAVcr-2; Hepatitis A virus cellular receptor 2; kidney injury molecule-3; sCD366; soluble CD366; soluble TIM 3; T cell immunoglobulin mucin 3; T-cell immunoglobulin and mucin domain 3; T-cell immunoglobulin and mucin domain-containing protein 3; T-cell immunoglobulin mucin family member 3; T-cell immunoglobulin mucin receptor 3; T-cell membrane protein 3; TIM-3; TIMD-3; unnamed protein product
Gene Aliases: CD366; HAVcr-2; HAVCR2; KIM-3; SPTCL; Tim-3; TIM3; TIMD-3; TIMD3
UniProt ID: (Human) Q8TDQ0
Entrez Gene ID: (Human) 84868
positive regulation of cytokine production
adaptive immune response
macrophage activation involved in immune response
immune system process
natural killer cell tolerance induction
regulation of tolerance induction dependent upon immune response
negative regulation of T-helper 1 type immune response
negative regulation of immune response to tumor cell
negative regulation of natural killer cell mediated cytotoxicity directed against tumor cell target
regulation of transcription from RNA polymerase II promoter
inflammatory response
negative regulation of gene expression
negative regulation of myeloid dendritic cell activation
negative regulation of NF-kappaB transcription factor activity
negative regulation of type I interferon production
negative regulation of interferon-alpha production
negative regulation of interferon-gamma production
negative regulation of interleukin-2 production
negative regulation of interleukin-3 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
positive regulation of chemokine production
positive regulation of interferon-gamma production
positive regulation of interleukin-1 production
positive regulation of interleukin-4 production
positive regulation of tumor necrosis factor production
negative regulation of natural killer cell activation
toll-like receptor 3 signaling pathway
toll-like receptor 7 signaling pathway
toll-like receptor 9 signaling pathway
positive regulation of T cell proliferation
negative regulation of T cell proliferation
positive regulation of macrophage activation
innate immune response
positive regulation of innate immune response
negative regulation of innate immune response
defense response to Gram-positive bacterium
maternal process involved in female pregnancy
positive regulation of ERK1 and ERK2 cascade
cellular response to lipopolysaccharide
negative regulation of granulocyte colony-stimulating factor production
negative regulation of defense response to bacterium
positive regulation of defense response to bacterium
positive regulation of NIK/NF-kappaB signaling
negative regulation of immunological synapse formation
negative regulation of T cell activation via T cell receptor contact with antigen bound to MHC molecule on antigen presenting cell
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Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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