Invitrogen

TIMP1 Monoclonal Antibody (F31 P2 A5)

Name: TIMP1 Monoclonal Antibody (F31 P2 A5)
Brand: Invitrogen
SKU: MA1-773

高级验证

2个已发表图片

12 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

TIMP1 Antibody in Immunocytochemistry (ICC/IF)

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TIMP1 Antibody (MA1-773) in ICC/IF

Immunofluorescence analysis of TIMP1 was done on 70% confluent log phase HepG2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with TIMP1 (F31 P2 A5) Mouse Monoclonal Antibody (Product # MA1-773) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175)... View More

TIMP1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Product Image Gallery

TIMP1 Antibody — Fig. 5 KDM3A activates the transcription of Timp1 in cardiomyocytes. Cardiomyocytes (CM) and cardiac fibroblasts (cFb) were isolated from WT and Kdm3a-Tg mouse hearts at week 6 post-Sham and TAC surgery. a - e Transcripts of fetal genes and fibrosis-related ECM genes were measured by qRT-PCR, normalized against internal Gapdh, and expressed relative to WT Sham CM. n = 3 +- SEM , * , # p < 0.05 by student t test. *, WT TAC vs WT Sham. # , Tg-TAC vs WT-TAC. f Cardiomyocytes from Kdm3a -Tg mouse hearts at week 6 post-Sham and TAC surgery were isolated and used for ChIP assay with antibodies against KDM3A (left panel) or H3K9me2 (right panel). The relative occupancies of KDM3A and H3K9me2 at Timp1 promoter were normalized against Input and expressed relative to Sham control. n = 3 +- SEM , *p < 0.05 ( t test). g Timp1-Luciferase reporter was transfected in combination with plasmids as indicate into 293 T cells. Cells were harvested 48 h after transfection. Luciferase activities were normalized against co-transfected beta-galactosidase, expressed relative to vector-transfected cells. n = 3 +- SEM. (h-k) Kdm3a- Tg mice were transduced with adenovirus AAV9 expressing control or Timp1 shRNA and subjected to Sham or TAC surgery. Hearts were echoed and harvested 5 weeks post-surgery. h WB of TIMP1 and GAPDH, i HW/BW, j %FS, and k percent of fibrotic area of Sham (S), TAC (T) Kdm3a-Tg mice transduced with control or Timp1 shRNA. n = 7 +- SEM, * p < 0.05 (ANOVA) {KD}

Immunogen image

Immunogen Image TIMP1 Monoclonal Antibody (F31 P2 A5)

产品信息

MA1-773

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:250-1:1,000

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

1:10-1:100

免疫细胞化学 (ICC/IF)

1-2 µg/mL

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染色质免疫沉淀实验 (ChIP)

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亲和纯化 (AP)

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产品规格

种属反应

Human, Mouse, Rhesus monkey

已发表种属

C. elegans, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

F31 P2 A5

抗原

Synthetic peptide corresponding to residues F(72) Q A L G D A A D I R(82) of mouse TIMP-1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_889482

产品详细信息

MA1-773 contains 100 µg of in vitro produced, protein A purified antibody (1 mg/mL) in PBS containing 1 mg/mL BSA and 0.05% sodium azide.

MA1-773 detects TIMP-1 from human samples.

MA1-773 has been successfully used in Western blot, immunofluorescence, and immunohistochemistry procedures. By Western blot, MA1-773 detects a ~30 kDa band representing TIMP-1 from K562 cell lysate. It is recommended to block with 3% BSA.

The MA1-773 immunogen is a synthetic peptide corresponding to residues F(72) Q A L G D A A D I R(82) of mouse TIMP-1.This sequence is 100% conserved in some non-human primate TIMP-1.

靶标信息

TIMP1 (metalloproteinase inhibitor 1) is a metalloproteinase inhibitor that functions by forms one-to-one complexes with target metallproteinases, such as collagenases, and irreversible inactivates them by binding to their catalytic zinc cofactor. TIMP1 acts on MMP1, MMP2, MMP3, MMP7, MMP8, MMP9, MMP10, MMP11, MMP12, MMP14, and MMP16. It also functions as a growth factor that regulates cell differenation, migration, and cell death. TIMP1 activates cellular signaling cascades via CD63 and ITGB1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: Collagenase inhibitor; Collagenase inhibitor 16C8 fibroblast; EPA; erythroid potentiating activity; Erythroid-potentiating activity; Fibroblast collagenase inhibitor; FLJ90373; Metalloproteinase inhibitor; Metalloproteinase inhibitor 1; MMP inhibitor; RP1-230G1.3; TIMP 1; TIMP-1; tissue inhibitor of matrix metalloproteinase-1; tissue inhibitor of metalloproteinase-1; Tissue inhibitor of metalloproteinases 1; TPA-induced protein; TPA-S1

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基因别名: CLGI; EPA; EPO; HCI; TIMP; TIMP-1; TIMP1; TPA-S1

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Entrez Gene ID: (Rhesus monkey) 574348, (Human) 7076, (Mouse) 21857

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功能

protease binding cytokine activity protein binding growth factor activity metalloendopeptidase inhibitor activity metal ion binding enzyme inhibitor activity peptidase inhibitor activity protease inhibitor

参与通路

cell activation platelet degranulation aging positive regulation of cell proliferation response to hormone negative regulation of endopeptidase activity extracellular matrix disassembly response to cytokine wound healing negative regulation of apoptotic process negative regulation of catalytic activity response to peptide hormone negative regulation of membrane protein ectodomain proteolysis cartilage development negative regulation of trophoblast cell migration regulation of integrin-mediated signaling pathway negative regulation of peptidase activity

TIMP1 Monoclonal Antibody (F31 P2 A5)

TIMP1 Antibody (MA1-773) in ICC/IF

产品信息

MA1-773

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

染色质免疫沉淀实验 (ChIP)

亲和纯化 (AP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

TIMP1 Monoclonal Antibody (F31 P2 A5)

TIMP1 Antibody (MA1-773) in ICC/IF

产品信息

MA1-773

免疫印迹 (WB)

免疫组化 (IHC)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

染色质免疫沉淀 实验 (ChIP)

亲和纯化 (AP)

种属反应

已发表种属

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

运输条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

随时为您服务

Your items have has been added!

染色质免疫沉淀实验 (ChIP)