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Invitrogen
TL1A Recombinant Mouse Monoclonal Antibody (Tandys1a), Alexa Fluor™ Plus 555
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TL1A Antibody (740170MP555) in ICC/IF
Immunofluorescence analysis of TL1A was performed using TL1A Recombinant Mouse Monoclonal Antibody (Tandys1a), Alexa Fluor™ Plus 555 (Product # 740170MP555) on HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 min, permeabilized with 0.1% Triton X-100 for 15 minutes and blocked with 2% BSA for 1 hr at RT. The cells were labeled with TL1A Recombinant Mouse Monoclonal Antibody (Tandys1a), Alexa Fluor™ Plus 555 (Product # 740170MP555, 1:100) in 0.1% BSA, incubated at 4°C. Panel a) shows representative image of cells that were s... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
740170MP555
产品规格
种属反应
Human
宿主/亚型
Mouse
/ IgG1, kappa
Expression System
Expi293
分类
Recombinant Monoclonal
类型
Antibody
克隆号
Tandys1a
偶联物
Alexa Fluor™ Plus 555
Alexa Fluor™ Plus 555
Alexa Fluor™ Plus 555
激发/发射光谱
558/572 nm
查看光谱
形式
Liquid
浓度
1 mg/mL
规格
50 µg
纯化类型
Protein A/G
保存液
proprietary buffer, pH 6.3-7.3
内含物
0.008% Bromonitrodioxane, 0.008% Methylisothiazolone
保存条件
4°C, store in dark, DO NOT FREEZE!
运输条件
Wet ice
产品详细信息
Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.
Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.
It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 553 nm; Emission: 568 nm; Laser: Yellow Laser
Filtration: 0.2 µm post-manufacturing filtered.
靶标信息
The protein encoded by this gene is a cytokine that belongs to the tumor necrosis factor (TNF) ligand family. This protein is abundantly expressed in endothelial cells, but is not expressed in either B or T cells. The expression of this protein is inducible by TNF and IL-1 alpha. This cytokine is a ligand for receptor TNFRSF25 and decoy receptor TNFRSF21/DR6. It can activate NF-kappaB and MAP kinases, and acts as an autocrine factor to induce apoptosis in endothelial cells. This cytokine is also found to inhibit endothelial cell proliferation, and thus may function as an angiogenesis inhibitor. An additional isoform encoded by an alternatively spliced transcript variant has been reported but the sequence of this transcript has not been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: cytokine; MGC129934; MGC129935; TNF ligand-related molecule 1; TNF superfamily ligand TL1A; tumor necrosis factor (ligand) superfamily, member 15; tumor necrosis factor ligand 1B; tumor necrosis factor superfamily member 15; unnamed protein product; vascular endothelial cell growth inhibitor; vascular endothelial growth inhibitor; vascular endothelial growth inhibitor-192A
基因别名: TL1; TL1A; TNLG1B; VEGI; VEGI192A
UniProt ID: (Human) O95150
Entrez Gene ID: (Human) 9966
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