Invitrogen

TLR2 Monoclonal Antibody (11G5)

Name: TLR2 Monoclonal Antibody (11G5)
Brand: Invitrogen
SKU: MA5-16260

高级验证

查看其他69个TLR2抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (4)
高级验证 (2)

TLR2 Antibody in Immunocytochemistry (ICC/IF)

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TLR2 Antibody (MA5-16260) in ICC/IF

Immunofluorescence analysis of TLR2 was performed using Raw 264.7 cells and Raw 264.7 treated with LPS (500 ng/mL, 6 hours). The cells were fixed with 4% paraformaldehyde for 10 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TLR2 Rat Monoclonal Antibody (Product # MA5-16260) at 1:100 dilution in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rat IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 488 (Product # A-11006) at a dilution of 1:2000 for 45 minutes a... View More

Product Image Gallery

TLR2 Antibody — Altered expression of target protein upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of TLR2 using TLR2 Monoclonal Antibody (Product # MA5-16260) shows increased expression of TLR2 protein in Raw 264.7 cell line upon treatment with LPS in membrane lipid rafts. {TM}

Immunogen image

Immunogen Image TLR2 Monoclonal Antibody (11G5)

产品信息

MA5-16260

应用

建议稀释比

已发表文章

免疫印迹 (WB)

4-6 µg/mL

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

2.5 µg/10^6 cells

产品规格

种属反应

Mouse

宿主/亚型

Rat / IgG2a, kappa

分类

Monoclonal

类型

Antibody

克隆号

11G5

抗原

A portion of amino acids 400-450 of murine TLR2.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2537777

产品详细信息

This monoclonal antibody only very weakly detects human TLR2 in Ramos cell lysate and is not recommended for human sample testing.

靶标信息

TLR2 is a member of the Toll-like receptor (TLR) family which play a fundamental role in pathogen recognition and activation of innate immunity. TLR proteins act through adaptor molecules such as MyD88 and TIRAP to activate various kinases and transcription factors such as Protein Kinase C (PKC) alpha/beta and NF-kappa-B. TLR2 can form heterodimers with either TLR1 or TLR6, and as a heterodimer, can recognize a variety of bacterial and mycoplasma lipoproteins respectively. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. The various TLRs exhibit different patterns of expression. TLR2 is expressed most abundantly in peripheral blood leukocytes, and mediates host response to Gram-positive bacteria and yeast via stimulation of NF-kappaB. TLR2 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. Ten human homologs of TLRs (TLR1-10) have been described. TLR2 is expressed in human cells such as tonsils, microglia, lymph nodes, and appendices, activated B-cells in germinal centers. CD14+ monocytes express the highest level of TLR2 followed by CD15+ granulocytes, CD19+ B-cells, and CD3+ T-cells. The expression of TLR2 on different cell types are regulated by different immune response modifiers. For example, LPS, GM-CSF, IL-1, and IL-10 up regulates TLR2 whereas IL-4, IFN-gamma, and TNF down regulate TLR2 expression in monocytes.

仅用于科研。不用于诊断过程。未经明确授权不得转售。