Fabgennix

TLR4 Polyclonal Antibody, FITC

查看其他64个TLR4 (CD284)抗体

问题与答案

问题与答案

问题与答案

产品信息

TLR4-FITC

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:1,000

-

酶联免疫吸附实验 (ELISA)

1:10,000

-

免疫沉淀 (IP)

1:50-1:250

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide taken within amino acid region 970-1000 on human TLR4 protein.

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱

形式

Liquid

浓度

0.5-1.5 mg/mL

纯化类型

Affinity chromatography

保存液

proprietary buffer, pH 7.4-7.8, with 30% glycerol, 0.5% BSA

内含物

0.02% sodium azide

保存条件

-20°C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

TLR4 is member of the Toll like receptor (TLR) family which plays a fundamental role in pathogen recognition and activation of innate immunity. TLRs are highly conserved from Drosophila to humans and share structural and functional similarities. This receptor is most abundantly expressed in placenta, and in myelomonocytic subpopulation of the leukocytes. Mammalian cells respond to LPS by activating TLR4. TLR4 belongs to the multi-protein complex of lipopolysaccharide (LPS) receptor, containing CD14, LY96 and TLR4, and is involved in signal transduction events induced by lipopolysaccharide (LPS) found in most gram-negative bacteria. TLR4 aids in the recognition of pathogen-associated molecular patterns (PAMPs) that are expressed on infectious agents, and mediate the production of cytokines necessary for the development of effective immunity. The various TLRs exhibit different patterns of expression. Mutations in the TLR4 gene have been associated with differences in LPS responsiveness. Also, several transcript variants of the TLR4 gene have been found, but the protein coding potential of most of them is uncertain. TLR4 is expressed by peripheral blood monocytes and a small population of B-cells and is also expressed in human placenta. Studies with TLR4-deficient mice indicate that the main ligand for TLR is lipopolysaccharide. Consequently, these mice also showed increased susceptibility to Gram-negative sepsis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: CD284; homolog of Drosophila toll; hToll; Toll-like receptor 4

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基因别名: ARMD10; CD284; TLR-4; TLR4; TOLL

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UniProt ID: (Human) D1CS53

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Entrez Gene ID: (Human) 7099

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lipopolysaccharide binding lipopolysaccharide receptor activity receptor activity transmembrane signaling receptor activity protein binding

activation of MAPK activity toll-like receptor signaling pathway B cell proliferation involved in immune response nitric oxide production involved in inflammatory response regulation of dendritic cell cytokine production MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway inflammatory response immune response I-kappaB kinase/NF-kappaB signaling I-kappaB phosphorylation positive regulation of platelet activation positive regulation of gene expression astrocyte development detection of fungus positive regulation of B cell proliferation lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide detection of lipopolysaccharide interferon-gamma production negative regulation of interferon-gamma production negative regulation of interleukin-17 production negative regulation of interleukin-23 production negative regulation of interleukin-6 production negative regulation of tumor necrosis factor production positive regulation of chemokine production positive regulation of interferon-alpha production positive regulation of interferon-beta production positive regulation of interferon-gamma production positive regulation of interleukin-1 production positive regulation of interleukin-10 production positive regulation of interleukin-12 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production positive regulation of tumor necrosis factor production negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 4 signaling pathway TRIF-dependent toll-like receptor signaling pathway T-helper 1 type immune response macrophage activation positive regulation of NF-kappaB import into nucleus positive regulation of tumor necrosis factor biosynthetic process defense response to bacterium positive regulation of interleukin-12 biosynthetic process innate immune response positive regulation of MHC class II biosynthetic process positive regulation of interferon-beta biosynthetic process positive regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of osteoclast differentiation positive regulation of transcription from RNA polymerase II promoter positive regulation of JNK cascade interleukin-1 beta secretion regulation of cytokine secretion positive regulation of inflammatory response defense response to Gram-negative bacterium positive regulation of NF-kappaB transcription factor activity positive regulation of nitric-oxide synthase biosynthetic process intestinal epithelial structure maintenance positive regulation of macrophage cytokine production necroptotic process negative regulation of ERK1 and ERK2 cascade positive regulation of ERK1 and ERK2 cascade positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to mechanical stimulus apoptotic signaling pathway positive regulation of NLRP3 inflammasome complex assembly