Invitrogen

TMEM127 Polyclonal Antibody

查看其他3个TMEM127抗体

问题与答案

问题与答案

问题与答案

抗体检测数据 (2)

TMEM127 Antibody in Immunocytochemistry (ICC/IF)

TMEM127 Antibody in Immunocytochemistry (ICC/IF)

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TMEM127 Antibody (PA5-144339) in ICC/IF

Immunocytochemistry analysis of A549 cells with TMEM127 polyclonal antibody (Product # PA5-144339) at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

TMEM127 Antibody in Immunocytochemistry (ICC/IF)

TMEM127 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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TMEM127 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry analysis of A549 cells with TMEM127 polyclonal antibody (Product # PA5-144339) at 1:133, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeabilized using 0.2% Triton X-100 and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Rabbit IgG (H+L).

TMEM127 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis with a TMEM127 polyclonal antibody (Product # PA5-144339) using a dilution of 1:400 and staining in paraffin-embedded human heart tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30 min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunogen image

TMEM127 Polyclonal Antibody

Immunogen Image TMEM127 Polyclonal Antibody

产品信息

PA5-144339

应用

建议稀释比

已发表文章

免疫组化（石蜡） (IHC (P))

1:200-1:500

-

免疫细胞化学 (ICC/IF)

1:50-1:200

-

酶联免疫吸附实验 (ELISA)

Assay-dependent

-

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant Human Transmembrane protein 127 protein (1-95AA)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

3.35 mg/mL

纯化类型

Protein G

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.03% Proclin 300

保存条件

-20°C or -80°C if preferred

运输条件

Ambient (domestic); Wet ice (international)

靶标信息

TMEM127 controls cell proliferation acting as a negative regulator of TOR signaling pathway mediated by mTORC1. May act as a tumor suppressor.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Transmembrane protein 127

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基因别名: TMEM127

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UniProt ID: (Human) O75204

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Entrez Gene ID: (Human) 55654

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molecular_function Rab GTPase binding

endosome organization negative regulation of cell proliferation negative regulation of TOR signaling