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          • Primary Antibodies ›
          • TNF alpha Antibodies

          Invitrogen

          TNF alpha Monoclonal Antibody (2TNF-H33), Biotin

          19 篇参考文献
          查看其他160个TNF alpha抗体

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          Cite TNF alpha Monoclonal Antibody (2TNF-H33), Biotin

          Additional Information:
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          • 抗体检测数据 (1)
          TNF alpha Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.
          TNF alpha Antibody in ELISA (ELISA)
          Group 53 Created with Sketch.

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          TNF alpha Antibody (M302B) in ELISA

          Sandwich ELISA analysis of Human TNF alpha Biotinylated MAb (Product # M302B) was performed using components of Thermo Fisher Scientific Human TNF alpha Colorimetric ELISA Kit (Product # EH3TNFA) by loading 50 µL per well of Recombinant Human TNF alpha in octuplicate at 1000, 500, 250, 125, 62.5, 31.2, 15.6, and 0 pg/mL across a 5 µg/mL rabbit anti-Human TNF pre-coated plate and incubated for 1 hour at room temperature. The plate was washed then incubated with 100 µl per well of Human TNF alpha Biotinylated MAb (Product # M302B) in duplica... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          TNF alpha Antibody in ELISA (ELISA)

          产品信息

          M302B

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          Assay-dependent
          -

          酶联免疫吸附实验 (ELISA)

          Assay-dependent
          查看18篇已发表文章 18篇已发表文章

          其他PubMed (Misc)

          -
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Human

          已发表种属

          Cynomolgus monkey, Human

          宿主/亚型

          Mouse / IgG1, kappa

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          2TNF-H33

          抗原

          Recombinant human TNF alpha

          偶联物

          Biotin Biotin Biotin
          • Unconjugated
          • Request custom conjugation

          形式

          Liquid

          浓度

          0.5 mg/mL

          纯化类型

          Protein G

          保存液

          PBS with 4% BSA

          内含物

          proprietary preservative

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_223561

          产品详细信息

          M302B targets TNF Alpha in ELISA, and WB applications and shows reactivity with Human samples. The M303 antibody (clone 2TNF-H34A) has successfully been paired as the coating antibody in a sandwich ELISA with detection antibody M302B (biotinylated conjugate of clone 2TNG-H33).



          The M302B immunogen is recombinant human TNF alpha.



          M302B detects TNF Alpha which has a predicted molecular weight of approximately 26 kDa.



          The M302B TNFa antibody (biotinylated conjugate of clone 2TNF-H33) has successfully been paired as the detection antibody in a sandwich ELISA with capture antibody M303 (clone 2TNF-H34A) Typical dilutions for sandwich ELISA include 1 µg/mL for coating and 0.125 - 0.25 µg/mL for detection.



          Biotinylated antibody M302B (clone 2TNF-H33) and antibody M303 (clone 2TNF-H34A) have successfully been used in combination with recombinant TNFa protein STNFA in ELISA applications.

          靶标信息

          TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: APC1 protein; Cachectin; cell apoptosis-related protein; DADB-70P7.1; TNF alpha; TNF superfamily; TNF superfamily member 2; TNF α; TNF, macrophage-derived; TNF, monocyte-derived; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor ligand 1F; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha; tumor necrotic factor alpha; unnamed protein product

          查看更多 收起

          基因别名: DIF; IMD127; TNF; TNF-alpha; TNFA; TNFSF2; TNLG1F

          查看更多 收起

          UniProt ID: (Human) P01375

          查看更多 收起

          Entrez Gene ID: (Human) 7124

          查看更多 收起

          功能
          transcription regulatory region sequence-specific DNA binding protease binding cytokine activity tumor necrosis factor receptor binding protein binding death receptor agonist activity identical protein binding receptor agonist activity
          参与通路
          negative regulation of transcription from RNA polymerase II promoter response to hypoxia microglial cell activation positive regulation of cytokine production positive regulation of protein phosphorylation negative regulation of endothelial cell proliferation negative regulation of L-glutamate transport macrophage activation involved in immune response chronic inflammatory response to antigenic stimulus regulation of immunoglobulin production positive regulation of immunoglobulin production negative regulation of cytokine production involved in immune response positive regulation of chronic inflammatory response to antigenic stimulus positive regulation of humoral immune response mediated by circulating immunoglobulin skeletal muscle contraction negative regulation of systemic arterial blood pressure glucose metabolic process defense response inflammatory response immune response humoral immune response JNK cascade JAK-STAT cascade circadian rhythm negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to xenobiotic stimulus response to mechanical stimulus response to virus response to bacterium response to salt stress response to fructose organ morphogenesis negative regulation of heart rate vascular endothelial growth factor production positive regulation of gene expression negative regulation of gene expression positive regulation of macrophage derived foam cell differentiation negative regulation of lipid storage response to activity regulation of metabolic process calcium-mediated signaling extracellular matrix organization osteoclast differentiation positive regulation of protein complex assembly positive regulation of defense response positive regulation of fever generation negative regulation of myelination response to nutrient levels response to lipopolysaccharide negative regulation of interleukin-6 production positive regulation of chemokine production positive regulation of fractalkine production positive regulation of interferon-gamma production positive regulation of interleukin-1 beta production positive regulation of interleukin-18 production positive regulation of interleukin-6 production positive regulation of interleukin-8 production tumor necrosis factor-mediated signaling pathway positive regulation of heterotypic cell-cell adhesion toll-like receptor 3 signaling pathway positive regulation of cellular amide metabolic process response to isolation stress response to macrophage colony-stimulating factor NIK/NF-kappaB signaling regulation of cell proliferation vasodilation defense response to bacterium positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of programmed cell death regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of protein complex disassembly positive regulation of protein complex disassembly positive regulation of MAP kinase activity positive regulation of MAPK cascade protein kinase B signaling positive regulation of JUN kinase activity positive regulation of neuron apoptotic process negative regulation of blood vessel endothelial cell migration negative regulation of viral genome replication innate immune response cellular extravasation positive regulation of nitric oxide biosynthetic process response to ethanol regulation of fat cell differentiation negative regulation of fat cell differentiation negative regulation of myoblast differentiation negative regulation of osteoblast differentiation regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of protein catabolic process positive regulation of action potential positive regulation of cell adhesion positive regulation of lipid metabolic process positive regulation of mitotic nuclear division negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated negative regulation of mitotic cell cycle positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation by iron negative regulation of glucose import positive regulation of JNK cascade astrocyte activation embryonic digestive tract development positive regulation of smooth muscle cell proliferation regulation of protein secretion regulation of inflammatory response positive regulation of inflammatory response negative regulation of neurogenesis regulation of developmental process regulation of insulin secretion modulation of synaptic transmission positive regulation of synaptic transmission regulation of synapse organization defense response to Gram-positive bacterium cognition leukocyte migration leukocyte tethering or rolling detection of mechanical stimulus involved in sensory perception of pain negative regulation of lipid catabolic process positive regulation of membrane protein ectodomain proteolysis regulation of secretion positive regulation of NF-kappaB transcription factor activity positive regulation of protein transport regulation of multicellular organismal process response to glucocorticoid positive regulation of hair follicle development positive regulation of protein kinase B signaling regulation of synaptic transmission, glutamatergic positive regulation of glial cell proliferation positive regulation of vitamin D biosynthetic process epithelial cell proliferation involved in salivary gland morphogenesis regulation of branching involved in salivary gland morphogenesis negative regulation of vascular wound healing negative regulation of branching involved in lung morphogenesis regulation of biological quality positive regulation of ERK1 and ERK2 cascade positive regulation of calcineurin-NFAT signaling cascade cellular response to molecule of bacterial origin cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to retinoic acid cellular response to nicotine cellular response to interferon-gamma cellular response to ionizing radiation positive regulation of mononuclear cell migration positive regulation of podosome assembly endothelial cell apoptotic process protein localization to plasma membrane negative regulation of oxidative phosphorylation inflammatory response to wounding apoptotic signaling pathway extrinsic apoptotic signaling pathway cellular response to toxic substance liver regeneration necroptotic signaling pathway negative regulation of bile acid secretion antiviral innate immune response response to Gram-negative bacterium positive regulation of neuroinflammatory response positive regulation of interleukin-33 production positive regulation of cytokine production involved in inflammatory response negative regulation of beta-amyloid clearance positive regulation of NIK/NF-kappaB signaling positive regulation of synoviocyte proliferation positive regulation of beta-amyloid formation response to L-glutamate positive regulation of neutrophil activation negative regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of pri-miRNA transcription from RNA polymerase II promoter positive regulation of protein localization to plasma membrane regulation of establishment of endothelial barrier negative regulation of bicellular tight junction assembly positive regulation of cellular protein localization cellular response to beta-amyloid positive regulation of vascular smooth muscle cell proliferation positive regulation of leukocyte adhesion to vascular endothelial cell positive regulation of leukocyte adhesion to arterial endothelial cell regulation of membrane lipid metabolic process response to 3,3',5-triiodo-L-thyronine response to gold nanoparticle positive regulation of protein localization to cell surface positive regulation of blood microparticle formation positive regulation of chemokine (C-X-C motif) ligand 2 production positive regulation of hepatocyte proliferation regulation of endothelial cell apoptotic process regulation of reactive oxygen species metabolic process positive regulation of DNA biosynthetic process negative regulation of apoptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
          It has to be done as per old AB suggested Products section.

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