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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TNF alpha Antibodies

          Invitrogen

          TNF alpha Monoclonal Antibody (TN3-19.12), Biotin, Functional Grade, eBioscience™

          7个已发表图片
          7 篇参考文献
          查看其他161个TNF alpha抗体

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          Cite TNF alpha Monoclonal Antibody (TN3-19.12), Biotin, Functional Grade, eBioscience™

          • 已发表图片 (7)
          TNF alpha Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          TNF alpha Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 7

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          TNF alpha Antibody (36-7423-85) in WB

          Neuropsychiatric disease and treatment 2022 - Published figure using TNF alpha monoclonal antibody (Product # 36-7423-85) in Western Blot {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          TNF alpha Antibody in Western Blot (WB)
          TNF alpha Antibody in Flow Cytometry (Flow)
          TNF alpha Antibody in ELISA (ELISA)
          TNF alpha Antibody in ELISA (ELISA)
          TNF alpha Antibody in ELISA (ELISA)
          TNF alpha Antibody in Neutralization (Neu)
          TNF alpha Antibody in Neutralization (Neu)

          产品信息

          36-7423-85

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          -
          查看1篇已发表文章 1篇已发表文章

          流式细胞分析 (Flow)

          -
          查看2篇已发表文章 2篇已发表文章

          酶联免疫吸附实验 (ELISA)

          -
          查看1篇已发表文章 1篇已发表文章

          中和实验 (Neu)

          -
          查看2篇已发表文章 2篇已发表文章

          功能检测 (Functional)

          Assay-Dependent
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Mouse, Rat

          已发表种属

          Mouse

          宿主/亚型

          Armenian hamster / IgG

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          TN3-19.12

          偶联物

          Biotin , Functional Grade Biotin , Functional Grade Biotin , Functional Grade

          形式

          Liquid

          浓度

          0.5 mg/mL

          纯化类型

          Affinity chromatography

          保存液

          PBS, pH 7.2

          内含物

          no preservative

          保存条件

          4°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_469765

          产品详细信息

          Description: The TN3-19.12 monoclonal antibody reacts with mouse, rat, and rabbit tumor necrosis factor-alpha (TNF-alpha), but not with human TNF-alpha. The TN3-19.12 antibody is a neutralizing antibody. mouse TNF-alpha is a 17 kDa factor produced by macrophages, monocytes, neutrophils, CD4+ T cells and NK cells. A 26 kDa form of TNF-alpha is expressed as a membrane-bound molecule. TNF-alpha is cytolytic and plays an important role in immune regulation. Dimers and trimers of TNF-alpha have been observed.

          Applications Reported: The Functional Grade Biotin anti-mouse TNF alpha antibody (clone TN3-19) has been reported for use as the in vivo capture antibody for the In Vivo Cytokine Capture Assay (IVCCA). The IVCCA facilitates measurement of cytokines in serum by increasing their in vivo half lives dramatically. This increases the sensitivity of measurement of in vivo cytokine production 30- to 1000- fold.

          For detailed methodology in this application, please refer to Finkelman, F., S. Morris, T. Orekhova, and D. Sehy. 2003. The In Vivo Cytokine Capture Assay for measurement of cytokine production in the mouse. In Current Protocols in Immunology. Unit 6.28. J. Coligan, A. Kruisbeek, D. Margulies, E. Shevach, and W. Strober, eds. John Wiley and Sons, New York The TN3-19.12 antibody has been reported for use in intracellular staining for flow cytometric analysis, cytokine neutralization, and ELISA. Fluorochrome conjugated TN3-19.12 is recommended for use in intracellular staining and flow cytometry. Functional Grade purified TN3-19.12, Product # 16-7423-81, is recommended for use in functional assays. For ELISPOT capture, the alternative clone, 1F3F3D4 (Product # 16-7325), is recommended.

          Applications Tested: The Functional Grade Biotin TN3-19 antibody has been tested by LAL assay to ensure it is free of endotoxin, and has been tested as the detection antibody in a sandwich ELISA for analysis of mouse TNF alpha in combination with the purified polyclonal anti-mouse TNF alpha antibody (Product # 14-7341-85) for capture and recombinant mouse TNF alpha (Product # 14-8321-62) as the standard. A suitable range of concentrations of the FG Biotin TN3-19 antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15.0 pg/mL should be included in each ELISA plate.

          Endotoxin: Less than 0.05 ng/µg antibody as determined by the LAL assay.

          Storage and handling: Use in a sterile environment.

          靶标信息

          TNF alpha is a multifunctional proinflammatory cytokine that belongs to the tumor necrosis factor (TNF) superfamily. This cytokine is mainly secreted by macrophage and bind to its receptors, TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. TNF alpha is involved in the regulation of immune cells, cell proliferation, differentiation, apoptosis, lipid metabolism, and coagulation. TNF alpha exists as a multimer of two, three, or five noncovalently linked units, but shows a single 17 kDa band following SDS-PAGE under non-reducing conditions. Knockout studies in mice also suggested the neuroprotective function of TNF alpha, and has been observed to causes tumor necrosis when injected into tumor-bearing mice. Other functions of TNF-alpha include its role in the immune response to bacterial, viral, parasitic and certain fungal infections, as well as its role in the necrosis of specific tumors. TNF alpha causes cytolysis or cytostasis of certain transformed cells, being synergistic with interferon-gamma in its cytotoxicity. This cytokine has been implicated in a variety of diseases, including autoimmune diseases, insulin resistance, and cancer.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: Cachectin; DADB-70P7.1; TNF alpha; TNF superfamily; TNF α; TNF-a; TNF-alpha; TNFα; Tumor necrosis factor; tumor necrosis factor (TNF superfamily, member 2); tumor necrosis factor alpha; Tumor necrosis factor ligand superfamily member 2; tumor necrosis factor-alpha

          查看更多 收起

          基因别名: DIF; RATTNF; Tnf; TNF-a; TNF-alpha; Tnfa; TNFalpha; Tnfsf1a; TNFSF2

          查看更多 收起

          UniProt ID: (Rat) P16599, (Mouse) P06804

          查看更多 收起

          Entrez Gene ID: (Rat) 24835, (Mouse) 21926

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          功能
          protease binding cytokine activity tumor necrosis factor receptor binding identical protein binding transcription regulatory region DNA binding protein binding
          参与通路
          protein import into nucleus, translocation negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPKKK activity activation of MAPK activity response to hypoxia cell activation positive regulation of cytokine production regulation of protein phosphorylation positive regulation of protein phosphorylation negative regulation of L-glutamate transport chronic inflammatory response to antigenic stimulus negative regulation of cytokine secretion involved in immune response positive regulation of chronic inflammatory response to antigenic stimulus positive regulation of humoral immune response mediated by circulating immunoglobulin skeletal muscle contraction glucose metabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process obsolete transformed cell apoptotic process defense response inflammatory response humoral immune response signal transduction JNK cascade multicellular organism development negative regulation of cell proliferation extrinsic apoptotic signaling pathway via death domain receptors intrinsic apoptotic signaling pathway in response to DNA damage response to radiation response to mechanical stimulus response to virus organ morphogenesis positive regulation of gene expression negative regulation of gene expression negative regulation of alkaline phosphatase activity response to activity calcium-mediated signaling extracellular matrix organization osteoclast differentiation sequestering of triglyceride positive regulation of protein complex assembly positive regulation of fever generation lipopolysaccharide-mediated signaling pathway response to lipopolysaccharide negative regulation of interleukin-6 production positive regulation of chemokine production positive regulation of interferon-gamma production positive regulation of interleukin-18 production positive regulation of interleukin-6 production receptor biosynthetic process positive regulation of peptidyl-serine phosphorylation tumor necrosis factor-mediated signaling pathway positive regulation of heterotypic cell-cell adhesion regulation of cell proliferation positive regulation of NF-kappaB import into nucleus response to drug defense response to bacterium positive regulation of apoptotic process positive regulation of programmed cell death regulation of I-kappaB kinase/NF-kappaB signaling positive regulation of I-kappaB kinase/NF-kappaB signaling negative regulation of protein complex disassembly positive regulation of protein complex disassembly positive regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity protein kinase B signaling positive regulation of JUN kinase activity positive regulation of neuron apoptotic process negative regulation of growth of symbiont in host negative regulation of viral genome replication positive regulation of chemokine biosynthetic process cellular extravasation positive regulation of interleukin-8 biosynthetic process positive regulation of nitric oxide biosynthetic process negative regulation of myoblast differentiation negative regulation of osteoblast differentiation regulation of osteoclast differentiation positive regulation of osteoclast differentiation positive regulation of action potential positive regulation of mitotic nuclear division positive regulation of protein kinase activity negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of translational initiation by iron negative regulation of glucose import positive regulation of JNK cascade embryonic digestive tract development positive regulation of smooth muscle cell proliferation regulation of protein secretion positive regulation of cytokine secretion positive regulation of inflammatory response positive regulation of phagocytosis regulation of insulin secretion positive regulation of synaptic transmission defense response to Gram-positive bacterium leukocyte migration leukocyte tethering or rolling detection of mechanical stimulus involved in sensory perception of pain negative regulation of lipid catabolic process regulation of immunoglobulin secretion positive regulation of membrane protein ectodomain proteolysis positive regulation of sequence-specific DNA binding transcription factor activity positive regulation of NF-kappaB transcription factor activity positive regulation of protein transport response to glucocorticoid positive regulation of NFAT protein import into nucleus positive regulation of hair follicle development positive regulation of protein kinase B signaling positive regulation of vitamin D biosynthetic process positive regulation of calcidiol 1-monooxygenase activity epithelial cell proliferation involved in salivary gland morphogenesis regulation of branching involved in salivary gland morphogenesis negative regulation of branching involved in lung morphogenesis cellular response to lipopolysaccharide cellular response to amino acid stimulus cellular response to nicotine cellular response to organic cyclic compound positive regulation of podosome assembly apoptotic signaling pathway extrinsic apoptotic signaling pathway necroptotic signaling pathway response to gold nanoparticle positive regulation of protein localization to cell surface positive regulation of chemokine (C-X-C motif) ligand 2 production regulation of reactive oxygen species metabolic process negative regulation of extrinsic apoptotic signaling pathway in absence of ligand immune response cell proliferation positive regulation of cell proliferation positive regulation of cell adhesion
          It has to be done as per old AB suggested Products section.
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