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Invitrogen
TNIK Polyclonal Antibody
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FIGURE: 1 / 8
TNIK Antibody (PA1-20639) in ICC/IF
Immunocytochemistry-Immunofluorescence analysis of TNIK was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: TNIK Polyclonal Antibody (Product # PA1-20639) diluted at 1:500. Red: alpha Tubulin, a cytoskeleton marker. Blue: Hoechst 33342 staining.
Product Details
PA1-20639
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Human,
Mouse
Published species
C. elegans
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Synthetic peptide. Exact sequence is proprietary.
Conjugate
Form
Liquid
Concentration
1 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
PBS
Contains
0.01% sodium azide
Storage conditions
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Suggested positive controls for this product are heart, skeletal muscle and brain.
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
Target Information
TNIK or TRAF2 and NCK interacting kinase is characterized by an N-terminal kinase domain and a C-terminal GCK domain that serves a regulatory function. TNIK is mainly expression in brain, heart, and spleen and it is a specific effector of RAP2 which regulate actin cytoskeleton. TNIK is autophosphorylated in a manner dependent upon lys54 in the ATP-binding pocket of its kinase domain and plays a main role in cytoskeleton regulation. TNIK has also been shown to phosphorylate gelsolin, the principal intracellular and extracellular actin-severing protein, in vitro. This and evidence from mutational studies suggest that TNIK functions in the regulation of the cytoskeleton.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Bioinformatics
Protein Aliases: TRAF2 and NCK-interacting protein kinase
Gene Aliases: 1500031A17Rik; 4831440I19Rik; AI451411; C530008O15Rik; C630040K21Rik; KIAA0551; TNIK
UniProt ID: (Human) Q9UKE5, (Mouse) P83510
Entrez Gene ID: (Human) 23043, (Mouse) 665113
protein kinase activity
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
protein binding
ATP binding
nucleotide binding
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
non-receptor serine/threonine protein kinase
positive regulation of protein phosphorylation
positive regulation of defense response to virus by host
protein phosphorylation
cytoskeleton organization
activation of JNKK activity
Wnt signaling pathway
microvillus assembly
actin cytoskeleton reorganization
intracellular signal transduction
protein autophosphorylation
regulation of dendrite morphogenesis
protein localization to plasma membrane
xenophagy
MAPK cascade
regulation of mitotic cell cycle
nervous system development
phosphorylation
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of apoptotic process
neuron projection morphogenesis
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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