Invitrogen

TOP2B Polyclonal Antibody

Name: TOP2B Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-54984

高级验证

查看其他8个TOP2B抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (9)
高级验证 (1)

TOP2B Antibody in Immunocytochemistry (ICC/IF)

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TOP2B Antibody (PA5-54984) in ICC/IF

Immunofluorescence analysis of TOP2B was performed using U-2 OS cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TOP2B Polyclonal Antibody (Product # PA5-54984) at 2.5 µg/mL concentration in 0.1% BSA and incubated overnight at 4 degree and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 (Product # A27034, 1:2000 diluti... View More

TOP2B Antibody in Immunohistochemistry (Paraffin) (IHC (P))

TOP2B Antibody in Immunohistochemistry (IHC)

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TOP2B Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. U2OS cells were transfected with, control siRNA, TOP2B siRNA probe #1, and TOP2B siRNA probe #2 and a decrease in signal intensity was observed in Western Blot application using Anti-TOP2B Polyclonal Antibody (Product # PA5-54984) used at a dilution of 0.4 µg/mL. Anti-GAPDH was used as a loading control. {KD}

Immunogen image

Immunogen Image TOP2B Polyclonal Antibody

产品信息

PA5-54984

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.04-0.4 µg/mL

免疫组化（石蜡） (IHC (P))

1:500-1:1,000

免疫细胞化学 (ICC/IF)

0.25-2 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein corresponding to Human TOP2B. Recombinant protein control fragment (Product #RP-92454).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.40 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.2, with 40% glycerol

内含物

0.02% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2648820

产品详细信息

Immunogen sequence: LWKEDLAAFV EELDKVESQE REDVLAGMSG KAIKGKVGKP KVKKLQLEET MPSPYGRRII PEITAMKADA SKKLLKKKKG DLDTAAVKVE FDEEFSGAPV EGAGEEALTP SVPINKGPKP KREKKEPGTR VRKTPTSSGK PSA

Highest antigen sequence identity to the following orthologs: Mouse - 89%, Rat - 40%.

靶标信息

TOP2B (DNA Topoisomerase II Beta) is a Protein Coding gene. This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, beta, is localized to chromosome 3 and the alpha form is localized to chromosome 17. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: antigen MLAA-44; DNA topoisomerase 2-beta; DNA topoisomerase II beta; DNA topoisomerase II, 180 kD; DNA topoisomerase II, beta isozyme; topo II beta; topoisomerase (DNA) II beta 180kDa; topoisomerase II beta; topoisomerase IIb; U937 associated antigen

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基因别名: D230016L12Rik; Top-2; TOP2B; top2beta; TOPIIB

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UniProt ID: (Human) Q02880, (Mouse) Q64511

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Entrez Gene ID: (Human) 7155, (Rat) 361100, (Mouse) 21974

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