Invitrogen

TPI1 Recombinant Rabbit Monoclonal Antibody (PSH14-38)

Name: TPI1 Recombinant Rabbit Monoclonal Antibody (PSH14-38)
Brand: Invitrogen
SKU: MA571363
Availability: InStock

高级验证

查看其他23个TPI1抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (20)
高级验证 (1)

TPI1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 21

TPI1 Antibody (MA571363) in ICC/IF

Immunofluorescence analysis of TPI1 was performed using SH-SY5Y cells. The cells were fixed with 4% paraformaldehyde for 5 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TPI1 Recombinant Rabbit Monoclonal Antibody (PSH14-38) (Product # MA571363) at 1:100 in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790, 1:2,0... View More

TPI1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

Product Image Gallery

TPI1 Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of the target protein. HEK-293 cells were transfected with TPI1 siRNA and a decrease in signal intensity was observed in Western blot application using TPI1 Recombinant Rabbit Monoclonal Antibody (PSH14-38) (Product # MA571363). {TM}

Immunogen image

Immunogen Image TPI1 Recombinant Rabbit Monoclonal Antibody (PSH14-38)

产品信息

MA571363

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:5,000

免疫组化（石蜡） (IHC (P))

1:1,000

免疫细胞化学 (ICC/IF)

1:100

流式细胞分析 (Flow)

1:1,000

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH14-38

抗原

Recombinant protein within human Triosephosphate isomerase aa 1-249.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Protein A

保存液

PBS, pH 7.4, with 40% glycerol, 0.1% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive Control: A431 cell lysate, A549 cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, COS-1 cell lysate, Mouse placenta tissue lysate, human colon tissue, human liver tissue, human placenta tissue, mouse colon tissue, mouse liver tissue, mouse placenta tissue, rat colon tissue, rat liver tissue, rat placenta tissue, A431, NIH/3T3, C6.

Tissue Specificity: Tissue enhanced (skeletal)

Subcellular Location: Cytoplasm, nucleus.

Sequence Similarities: 94% Mouse/Rat

Predicted band size: 27 kDa

靶标信息

This gene encodes an enzyme, consisting of two identical proteins, which catalyzes the isomerization of glyceraldehydes 3-phosphate and dihydroxy-acetone phosphate in glycolysis and gluconeogenesis. Mutations in this gene are associated with triosephosphate isomerase deficiency. Pseudogenes have been identified on chromosomes 1, 4, 6 and 7. Alternative splicing results in multiple transcript variants.

仅用于科研。不用于诊断过程。未经明确授权不得转售。