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TPL2 Antibody (710377) in ICC/IF
Immunofluorescence analysis of COT/TPL2/MAP3K8 was done on 70% confluent log phase U-87MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with COT/TPL2/MAP3K8 (3HCLC), Recombinant Rabbit Superclonal™ Antibody (Product # 710377) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alex... View More
产品信息
710377
产品规格
种属反应
Human,
Mouse
已发表种属
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
3HCLC
抗原
Peptide corresponding to amino acids 282-293 of human COT/TPL2/MAP3K8
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
产品详细信息
This antibody is predicted to react with bovine, canine, equine, mouse, rabbit and rat based on sequence homology.
Recombinant rabbit Superclonal™ antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit Superclonal™ antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production. Note: Formerly called “Recombinant polyclonal antibody”, this product is now rebranded as “Recombinant Superclonal™ antibody”. The physical product and the performance remain unchanged.
靶标信息
MAP3K8 (COT) is a serine/threonine kinase which can activate both the MAP and JNK kinase pathways and plays a role in the cell cycle. Mitogen-activated protein kinase (MAPK) signaling cascades include MAPK or extracellular signal-regulated kinase (ERK), MAPK kinase (MKK or MEK), and MAPK kinase (MAPKKK or MEKK). MAPKK kinase/MEKK phosphorylates and activates its downstream protein kinase, MAPK kinase/MEK, which in turn activates MAPK. The kinases of these signaling cascades are highly conserved, and homologs exist in yeast, Drosophila, and mammalian cells. MEKK8 is able to activate NF-kappa-B 1 by stimulating proteasome-mediated proteolysis of NF-kappa-B 1/p105. This cytoplasmic protein is expressed in several normal tissues and human tumor-derived cell lines. The 58 kDa form is activated specifically during the S and G2/M phases of the cell cycle. The longer form undergoes phosphorylation on Ser residues mainly, and the shorter form on both Ser and Thr residues.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: augmented in rheumatoid arthritis 2; Cancer Osaka thyroid oncogene; cancer Osaka thyroid, oncogene; CCOT; cot (cancer Osaka thyroid) oncogene; COT proto-oncogene serine/threonine-protein kinase; EC 1.14.16.2; Ewing sarcoma transformant; FLJ10486; M3K8; MAP3K 8; mitogen activated protein kinase kinase kinase 8; Mitogen-activated protein kinase kinase kinase 8; Protein kinase cot; Proto-oncogene c-Cot; proto-oncogene serine/threoine protein kinase; Serine/threonine-protein kinase cot; TH isoform 3; TH isoform a; TH-4; TPL 2; TPL-2; Tumor progression locus 2; TY3H; TYH
基因别名: AURA2; c-COT; COT; Cot/Tpl2; EST; ESTF; MAP3K8; MEKK8; Tpl-2; TPL2
UniProt ID: (Human) P41279, (Mouse) Q07174
Entrez Gene ID: (Human) 1326, (Mouse) 26410
magnesium ion binding
protein serine/threonine kinase activity
receptor signaling protein serine/threonine kinase activity
MAP kinase kinase kinase activity
protein binding
ATP binding
nucleotide binding
protein kinase activity
kinase activity
transferase activity
transferase activity, transferring phosphorus-containing groups
metal ion binding
non-receptor serine/threonine protein kinase
activation of MAPKK activity
protein phosphorylation
apoptotic process
cell cycle
T cell costimulation
stress-activated MAPK cascade
immune system process
signal transduction
regulation of mitotic cell cycle
phosphorylation
signal transduction by protein phosphorylation
stress-activated protein kinase signaling cascade
activation of protein kinase activity
regulation of apoptotic process
