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Invitrogen
TPPP Polyclonal Antibody
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FIGURE: 1 / 1
TPPP Antibody (PA5-117141) in WB
Western blot analysis of TPPP in extracts of various cell lines. Samples were incubated with TPPP Polyclonal antibody (Product # PA5-117141) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-117141
Product Specifications
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-219 of human TPPP/p25 (NP_0089611)
Conjugate
Form
Liquid
Concentration
0.94 mg/mL
Amount
94 µg
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.3, with 50% glycerol
Contains
0.02% sodium azide
Storage conditions
-20°C, Avoid Freeze/Thaw Cycles
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
Positive Samples: Jurkat, Mouse brain, Mouse pancreas, Rat brain
Immunogen sequence: MADKAKPAKA ANRTPPKSPG DPSKDRAAKR LSLESEGAGE GAAASPELSA LEEAFRRFAV HGDARATGRE MHGKNWSKLC KDCQVIDGRN VTVTDVDIVF SKIKGKSCRT ITFEQFQEAL EELAKKRFKD KSSEEAVREV HRLIEGKAPI ISGVTKAISS PTVSRLTDTT KFTGSHKERF DPSGKGKGKA GRVDLVDESG YVSGYKHAGT YDQKVQGGK
Target Information
Tubulin family members are globular proteins important in the assembly of microtubules. Microtubules are structural components that play important roles in mitosis, cytokinesis and vesicle transport. TPPP (Tubulin polymerization-promoting protein), also known as p24 and p25, is a widely expressed 219 amino acid protein found in the perinuclear region of the cytoplasm. TPPP may form dimers and functions in polymerizing tubulin into double-walled tubules, polymorphic aggregates, or stabilized blocks. TPPP overexpression prevents formation of the mitotic spindle assembly and breakdown of the nuclear envelope. TPPP is phosphorylated by TPK II and is encoded by a gene that maps to human chromosome 5, which contains 181 million base pairs and comprises nearly 6% of the human genome. May play a role in the polymerization of tubulin into microtubules, microtubule bundling and the stabilization of existing microtubules, thus maintaining the integrity of the microtubule network. May play a role in mitotic spindle assembly and nuclear envelope breakdown.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: 25 kDa brain-specific protein; brain specific protein p25 alpha; brain-specific protein p25 alpha; glycogen synthase kinase 3 (GSK3) inhibitor p24; p24; p25-alpha; TPPP; TPPP/p25; transcription factor; Tubulin polymerization-promoting protein; unnamed protein product
Gene Aliases: 2900041A09Rik; AI849835; p24; p25; p25alpha; RGD1310121; TPPP; TPPP/p25; TPPP1
UniProt ID: (Human) O94811, (Rat) D3ZQL7, (Mouse) Q7TQD2
Entrez Gene ID: (Human) 11076, (Rat) 361466, (Mouse) 72948
microtubule bundle formation
oligodendrocyte development
astral microtubule organization
positive regulation of protein complex assembly
positive regulation of myelination
positive regulation of protein polymerization
myelin assembly
microtubule polymerization
oligodendrocyte differentiation
cell division
microtubule nucleation by microtubule organizing center
regulation of microtubule cytoskeleton organization
negative regulation of tubulin deacetylation
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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