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Invitrogen
TRIM21 Polyclonal Antibody
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图: 1 / 11
TRIM21 Antibody (PA5-22294) in ICC/IF
Immunofluorescence analysis of TRIM21 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with TRIM21 Polyclonal Antibody (Product # PA5-22294) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A3279... View More
产品信息
PA5-22294
应用
建议稀释比
已发表文章
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human SSA1. The exact sequence is proprietary.
偶联物
形式
Liquid
浓度
1.52 mg/mL
规格
152 µg
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: 293T, A431, H1299, HeLa, HepG2, Molt-4, Raji, TRIM21-transfected 293T.
Predicted reactivity: Cat (86%), Pig (81%), Rhesus Monkey (99%), Bovine (82%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
This gene encodes a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The encoded protein is part of the RoSSA ribonucleoprotein, which includes a single polypeptide and one of four small RNA molecules. The RoSSA particle localizes to both the cytoplasm and the nucleus. RoSSA interacts with autoantigens in patients with Sjogren syndrome and systemic lupus erythematosus. Alternatively spliced transcript variants for this gene have been described but the full-length nature of only one has been determined.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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