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Invitrogen
TRIM50 Polyclonal Antibody
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TRIM50 Antibody (PA5-144038) in WB
Western blot analysis of TRIM50 using TRIM50 antibody (Product # PA5-144038). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 µg of sample under reducing conditions. Lane 1: human HepG2 whole cell lysates; Lane 2: human K562 whole cell lysates; Lane 3: human 293T whole cell lysates; Lane 4: human U-87MG whole cell lysates. After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. ... View More
产品信息
PA5-144038
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
E.coli-derived human TRIM50 recombinant protein (Position: K243-L487).
偶联物
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
no preservative
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.
Positive Control - WB: human HepG2 whole cell, human K562 whole cell, human 293T whole cell, human U-87MG whole cell. Flow: CACO-2 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.
靶标信息
TRIM50 belongs to the TRIM/RBCC family. It contains 1 B box-type zinc finger, 1 B30.2/SPRY domain and 1 RING-type zinc finger. TRIM50 is an E3 ubiquitin-protein ligase.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: E3 ubiquitin-protein ligase TRIM50; RING-type E3 ubiquitin transferase TRIM50; tripartite motif-containing 50A; Tripartite motif-containing protein 50
基因别名: TRIM50; TRIM50A
UniProt ID: (Human) Q86XT4
Entrez Gene ID: (Human) 135892
