Invitrogen

TRIM6 Polyclonal Antibody

Name: TRIM6 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-143867

查看其他3个TRIM6抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (7)

TRIM6 Antibody in Immunocytochemistry (ICC/IF)

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TRIM6 Antibody (PA5-143867) in ICC/IF

Immunocytochemistry analysis of TRIM6 in U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. Samples were then incubated in TRIM6 Polyclonal antibody (Product # PA5-143867) using a dilution of 2 μg/mL. DyLight®488 conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate f... View More

TRIM6 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image TRIM6 Polyclonal Antibody

产品信息

PA5-143867

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human TRIM6 recombinant protein (Position: Q50-H349).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_3075081

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: human K56 whole cell, human HEK293 whole cell, human A549 whole cell, rat thymus tissue, mouse thymus tissue. IHC: human tonsil tissue, mouse thymus tissue, rat spleen tissue. ICC/IF: U20S cell. Flow: 293T cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

TRIM6 is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the nucleus, but its specific function has not been identified.The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. The protein localizes to the nucleus, but its specific function has not been identified. This gene is mapped to chromosome 11p15, where it resides within a TRIM gene cluster. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. A read-through transcript transcribed from this gene and TRIM34 has been observed.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: RING finger protein 89; RING-type E3 ubiquitin transferase TRIM6; tripartite motif protein 6; tripartite motif-containing 6; Tripartite motif-containing protein 6

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基因别名: C430046K18Rik; D7Ertd684e; RNF89; TRIM6

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UniProt ID: (Human) Q9C030, (Mouse) Q8BGE7

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Entrez Gene ID: (Human) 117854, (Rat) 293294, (Mouse) 94088

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