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Invitrogen
TRPA1 Polyclonal Antibody, DyLight™ 650
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FIGURE: 1 / 1
TRPA1 Antibody (PA5-22832) in Flow
Flow cytometry analysis of TRPA1 in A549 cells. Samples were incubated with TRPA1 polyclonal antibody (Product # PA5-22832) using a dilution of 2.5 µg/mL for 30 minutes at room temperature. An intracellular stain was performed with primary antibody (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin.
Product Details
PA5-22832
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Guinea pig,
Human,
Mouse,
Rat,
Zebrafish
Host/Isotype
Rabbit
/ IgG
Class
Polyclonal
Type
Antibody
Immunogen
A synthetic peptide made to a region within the N-terminus (residues 1-100) of the human TRPA1 protein.
Conjugate
Excitation/Emission Max
651/673 nm
View spectra
Form
Liquid
Concentration
0.63 mg/mL
Purification
Antigen affinity chromatography
Storage buffer
50mM sodium borate
Contains
0.05% sodium azide
Storage conditions
4°C, store in dark
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
Product Specific Information
The target sequence has 69% sequence homology with rat.
Suggested positive control: human brain membrane fraction.
Target Information
TRPA1 is a TRP-related channel that responds to cold temperatures and pungent compounds and plays a role in both nociceptor and hair cell transduction. TRPA1 is a transformation -associated gene product in lung epithelia, whereas its protein distribution is primarily restricted to sensory neurons. Blocking TRPA1 may be a therapeutic target for treating cold hyperalgesia caused by inflammation and nerve damage. It is now known that TRPA1 protein is also widely expressed outside of the CNS and is dys-regulated during oncogenic transformation. The structure of TRPA1 is highly related to both the protein ankyrin and transmembrane proteins. The specific function of TRPA1 has not yet been determined; however, studies indicate the function may involve a role in signal transduction and growth control.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ANKTM1; ankyrin-like with transmembrane domains 1; Ankyrin-like with transmembrane domains protein 1; ion channel; drTRPA1; nompC; p120; Transformation-sensitive protein p120; transient receptor potential A1; Transient receptor potential cation channel subfamily A member 1; transient receptor potential cation channel, subfamily A, member 1; TRPA-1; Wasabi receptor
Gene Aliases: ANKTM1; FEPS; TRPA1; TRPA1b
UniProt ID: (Human) O75762, (Mouse) Q8BLA8, (Rat) Q6RI86
Entrez Gene ID: (Human) 8989, (Mouse) 277328, (Guinea pig) 100526649, (Rat) 312896, (Zebrafish) 474351
ion transport
cell surface receptor signaling pathway
response to cold
response to organic substance
response to organic cyclic compound
sensory perception of pain
response to drug
response to hydrogen peroxide
response to pain
thermoception
detection of mechanical stimulus involved in sensory perception of pain
detection of chemical stimulus involved in sensory perception of pain
release of sequestered calcium ion into cytosol
protein homotetramerization
calcium ion transmembrane transport
transport
calcium ion transport
ion transmembrane transport
response to stimulus
detection of chemical stimulus
detection of temperature stimulus involved in thermoception
detection of mechanical stimulus involved in sensory perception
transmembrane transport
response to fatty acid
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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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