Invitrogen

TSC1 Polyclonal Antibody

Name: TSC1 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-119858

查看其他22个TSC1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (7)

TSC1 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 7

TSC1 Antibody (PA5-119858) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of of Hamartin in LOVO cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with TSC1 Polyclonal Antibody (Product # PA5-119858) at a dilution of 1:100 for 1 hour at room temperature, washed with PBS. Alexa Fluor 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1:1,000 dilution. The nuclear counter stain is DAPI (blue).

TSC1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Immunogen image

Immunogen Image TSC1 Polyclonal Antibody

产品信息

PA5-119858

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:100-1:500

免疫细胞化学 (ICC/IF)

1:100-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein within human Hamartin aa 400-600

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

TBS, pH 7.4, with 50% glycerol, 0.2% BSA

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2913430

产品详细信息

Positive Control: LO2 cell lysate, 293 cell lysate, A431 cell lysate, mouse pancreas tissue lysate, rat brain tissue lysate, LOVO, rat heart tissue, human fetal skeletal muscle tissue, human placenta tissue, Hela cell lysates.

Subcellular Location: Cytoplasm, Membrane.

靶标信息

Tuberous sclerosis complex (TSC) is an autosomal dominant tumor syndrome caused by mutations in either of the TSC1 or TSC2 tumor suppressor genes. The products of these genes form a protein complex that indirectly decreases the signaling of the mammalian Target of Rapamycin (TOR), an evolutionarily conserved serine/threonine kinase that regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. TOR activity is stimulated by Rheb, a member of the Ras superfamily of G-proteins, when the GTP/GDP ratio bound to Rheb is high. Immunoprecipitated TSC1/TSC2 has been shown to stimulate Rheb GTPase activity in vitro, suggesting that the TSC1/TSC2 decreases the ability of Rheb to stimulate TOR activity. This is supported by experiments showing overexpression of TSC1 and TSC2 results in a significant decrease in the GTP/GDP ratio bound to Rheb and the inhibition of cell growth. A shorter 40 kDa isoform of TSC1 has been shown to exist but its function is unknown.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Hamartin; HGNC:12362; MGC86987; OTTHUMP00000022439; OTTHUMP00000198378; Tuberous sclerosis 1 protein; Tuberous sclerosis 1 protein homolog; tumor suppressor

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基因别名: KIAA0243; LAM; TSC; TSC1

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UniProt ID: (Human) Q92574, (Rat) Q9Z136, (Mouse) Q9EP53

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Entrez Gene ID: (Human) 7248, (Rat) 60445, (Mouse) 64930

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功能

protein binding GTPase regulator activity GTPase activating protein binding protein N-terminus binding chaperone binding

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