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Invitrogen
TTL Polyclonal Antibody
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TTL Antibody (PA5-87576) in WB
Western blot analysis of TTL in extracts of various cell lines. Samples were incubated with TTL Polyclonal antibody (Product # PA5-87576) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
产品信息
PA5-87576
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human TTL (NP_7149231)
偶联物
形式
Liquid
浓度
1.44 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Immunogen sequence: MYTFVVRDEN SSVYAEVSRL LLATGHWKRL RRDNPRFNLM LGERNRLPFG RLGHEPGLVQ LVNYYRGADK LCRKASLVKL IKTSPELAES CTWFPESYVI YPTNLKTPVA PAQNGIQPPI SNSRTDEREF FLASYNRKKE DGEGNVWIAK SSAGAKGEGI LISSEASELL DFIDNQGQVH VIQKYLEHPL LLEPGHRKFD IRSWVLVDHQ YNIYLYREGV LRTASEPYHV DNFQDKTCHL TNHCIQKEYS KNYGKYEEGN EMFFKEFNQY; Positive Samples: SH-SY5Y, HeLa, SKOV3, Mouse brain, Mouse lung, Mouse heart, Rat kidney
靶标信息
Catalyzes the post-translational addition of a tyrosine to the C-terminal end of detyrosinated alpha-tubulin.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 2410003M22Rik; TTL; Tubulin--tyrosine ligase; tubulin-tyrosine ligase
基因别名: 2410003M22Rik; 2700049H19Rik; AI848570; TTL
UniProt ID: (Human) Q8NG68, (Mouse) P38585, (Rat) Q9QXJ0
Entrez Gene ID: (Human) 150465, (Mouse) 69737, (Rat) 171572
