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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • TUBA1A Antibodies

          Invitrogen

          TUBA1A Polyclonal Antibody

          9个已发表图片
          13 篇参考文献
          查看其他5个TUBA1A抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite TUBA1A Polyclonal Antibody

          • 抗体检测数据 (12)
          • 已发表图片 (9)
          TUBA1A Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          TUBA1A Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 21

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          TUBA1A Antibody (PA5-29444) in ICC/IF

          Immunofluorescence analysis of alpha Tubulin was performed using 70% confluent log phase NIH/3T3 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with Alpha Tubulin Rabbit Polyclonal Antibody (Product # PA5-29544) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 co... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          TUBA1A Antibody in Immunocytochemistry (ICC/IF)
          TUBA1A Antibody in Immunocytochemistry (ICC/IF)
          TUBA1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TUBA1A Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)
          TUBA1A Antibody in Western Blot (WB)

          产品信息

          PA5-29444

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:500-1:20,000
          查看11篇已发表文章 11篇已发表文章

          免疫组化(石蜡) (IHC (P))

          1:100-1:1,000
          -

          免疫组化(冰冻) (IHC (F))

          Assay-dependent
          -

          免疫细胞化学 (ICC/IF)

          1:100-1:1,000
          查看2篇已发表文章 2篇已发表文章
          产品规格

          种属反应

          Chicken, Fruit fly, Human, Insect, Mouse, Non-human primate, Protozoa, Rat

          已发表种属

          Human, Mouse

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          Recombinant protein encompassing a sequence within the center region of human alpha Tubulin. The exact sequence is proprietary.

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1.92 mg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS, pH 7, with 20% glycerol

          内含物

          0.025% ProClin 300

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2546920

          产品详细信息

          Recommended positive controls: 293T, A431, HeLa, HepG2, Jurkat, Raji, HeLa cytosol fraction, Neuro 2A, C8D30, NIH-3T3, Raw264.7, C2C12, PC-12, Rat-2, Drosophila.

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          靶标信息

          Alpha Tubulin is a part of a heterodimer consisting of alpha and beta tubulin subunits. The alpha and beta tubulins, which are each about 55 kDa MW, are homologous but not identical. Alpha-beta tubulin heterodimer is the basic building block of microtubules, and this intracellular cylindrical filamentous structure is present in almost eukaryotic cells. Microtubules serve as structural supports and lines of transport within the cell, as well as serving a key role in mitosis. Microtubules of the eukaryotic cytoskeleton perform essential and diverse functions and are composed of a heterodimer of alpha and beta tubulins. The alpha and beta tubulins represent the major components of microtubules, while gamma tubulin plays a critical role in the nucleation of microtubule assembly. There are multiple alpha and beta tubulin genes, which are highly conserved among species. The gene that encodes alpha tubulin is highly similar to the mouse and rat Tuba1 gene. Northern blotting studies have shown that the gene expression is predominantly found in morphologically differentiated neurologic cells.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: alpha 1a antibody; alpha 3 antibody; Alpha-tubulin 1; Alpha-tubulin 3; alpha-tubulin 3 antibody; Alpha-tubulin isotype M-alpha-1; B-ALPHA-1 antibody; brain-specific antibody; FLJ25113 antibody; FLJ30169; H2 ALPHA; H2-ALPHA; H2ALPHA; hum-a-tub1; hum-a-tub1 antibody; hum-a-tub2; hum-a-tub2 antibody; LIS3 antibody; M40; MGC117247; MGC16435; OK/SW cl.56; OK/SW-cl.56; OK/SWcl.56; Talpha1 alpha-tubulin; TUBA1A antibody; TUBA3 antibody; TUBB1; TUBB5; Tubulin alpha-1 chain; Tubulin alpha-1A chain; tubulin alpha-1A chain antibody; Tubulin alpha-3 chain; tubulin alpha-3 chain antibody; Tubulin B-alpha-1; tubulin B-alpha-1 antibody; tubulin, alpha 1; tubulin, alpha 1 (testis specific); tubulin, alpha 1a; tubulin, alpha 6; tubulin, alpha, brain-specific; unnamed protein product

          查看更多 收起

          基因别名: B-ALPHA-1; LIS3; Tuba-1; TUBA1; TUBA1A; TUBA3; TUBA6

          查看更多 收起

          UniProt ID: (Human) Q71U36, (Mouse) P68369, (Rat) P68370

          查看更多 收起

          Entrez Gene ID: (Chicken) 429035, (Human) 7846, (Mouse) 22142, (Rat) 64158

          查看更多 收起

          功能
          GTPase activity structural constituent of cytoskeleton GTP binding structural molecule activity protein binding protein domain specific binding nucleotide binding protein heterodimerization activity tubulin
          参与通路
          microtubule cytoskeleton organization mitotic cell cycle microtubule-based process G2/M transition of mitotic cell cycle cytoskeleton organization cytoskeleton-dependent intracellular transport cell division protein polymerization metabolic process
          It has to be done as per old AB suggested Products section.
          guarantee_icon

          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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