Invitrogen

Talin 1 Recombinant Rabbit Monoclonal Antibody (PSH09-95)

Name: Talin 1 Recombinant Rabbit Monoclonal Antibody (PSH09-95)
Brand: Invitrogen
SKU: MA562909

高级验证

查看其他21个Talin 1抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (5)
高级验证 (4)

Talin 1 Antibody in Immunocytochemistry (ICC/IF)

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Talin 1 Antibody (MA562909) in ICC/IF

Immunofluorescence analysis of Talin 1 was performed using HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Talin 1 Recombinant Rabbit Monoclonal Antibody (PSH09-95) (Product # MA562909) at 1:100 dilution in 0.1% BSA, incubated at 4 degree Celsius overnight and then labeled with Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 488 (Product # A32790), (1:2,000 dilution), for 45 minutes at room te... View More

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Immunogen image

Immunogen Image Talin 1 Recombinant Rabbit Monoclonal Antibody (PSH09-95)

产品信息

MA562909

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:2,000

免疫细胞化学 (ICC/IF)

1:100

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

Expression System

HEK293 cells

分类

Recombinant Monoclonal

类型

Antibody

克隆号

PSH09-95

抗原

Recombinant protein within human Talin 1 aa 1,700-2,000/2,541.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.4, with 0.1% BSA, 40% glycerol

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Positive Control: HeLa cell lysate, HUVEC cell lysate, HepG2 cell lysate, MEF cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, C6 cell lysate.

Tissue Specificity: Low tissue specificity.

Subcellular Location: Cell projection, Cytoplasm, Cell surface, Cell junction.

Sequence Similarities: 98% Mouse/Rat.

Predicted band size: 270 kDa

靶标信息

Talin, a multifunctional constituent of cell-substratum attachment sites, is a high molecular weight protein (225-235 kDa) found in variety of tissues and cell types. It is localized at a subset of adherens junctions, specialized cell-cell and cell-matrix associations that are characterized by the presence of filamentous actin at the cytoplasmic face of the junctional complex. In cultured cells, talin is absent from cell-cell junctions and found predominantly at adhesion plaques and in fibrillar streaks underlying cell surface fibronectin. Talin interacts with at least two other proteins that are localized at adhesion plaques, vinculin and integrin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。