Invitrogen

Tau Monoclonal Antibody (T46)

Name: Tau Monoclonal Antibody (T46)
Brand: Invitrogen
SKU: 13-6400

高级验证

25个已发表图片

59 篇参考文献

查看其他137个Tau抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Tau Antibody in Immunocytochemistry (ICC/IF)

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Tau Antibody (13-6400) in ICC/IF

Immunofluorescent analysis of Tau was done on 70% confluent log phase SHSY5Y cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Tau Mouse Monoclonal Antibody (Product # 13-6400) at 1:250 dilution in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Rabbit Anti-Mouse IgG Secondary Antibody (Product # A-11059) at a dilution of 1:400 for 30 minutes... View More

Tau Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Tau Antibody in Immunohistochemistry (Frozen) (IHC (F))

Tau Antibody in Immunohistochemistry (IHC)

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Tau Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across Mouse Brain, Rat Brain, Mouse Kidney, Mouse Lung, Rat Lung owing to their inherent genetic constitution. Relative expression of Tau was observed in Mouse and Rat Brain as compared to Kidney or Lung tissues using Anti-Tau Monoclonal Antibody (T46) (Product # 13-6400) in Western Blot. {RE}

产品信息

13-6400

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5-1.0 µg/mL

查看30篇已发表文章 30篇已发表文章

免疫组化 (IHC)

查看9篇已发表文章 9篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

1:250

查看14篇已发表文章 14篇已发表文章

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

2-5 µg

查看1篇已发表文章 1篇已发表文章

点杂交 (DB)

查看1篇已发表文章 1篇已发表文章

免疫电镜技术 (IM)

Assay-dependent

亲和纯化 (AP)

查看2篇已发表文章 2篇已发表文章

其他PubMed (Misc)

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产品规格

种属反应

Bovine, Chicken, Human, Mouse, Rabbit, Rat, Xenopus

已发表种属

C. elegans, Fruit fly, Human, Mouse, Non-human primate, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

T46

抗原

Bovine Tau.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

purified

保存液

PBS

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533025

产品详细信息

This antibody recognizes an epitope near the C-terminal region (404-441 aa) of human tau. It reacts with all 7 isoforms of human tau (binding-competent and binding-incompetent), A68 (an abnormal phosphorylated derivative of tau), bovine tau, and human NeuroFibrillary Tangles (NFTs).

Reactivity is independent of the state of tau phosphorylation. Some cross-reactivity with MAP2 is also observed.

This antibody is suitable for immunohistochemical staining of Bouin's-fixed or alcohol-fixed, paraffin-embedded or frozen tissue sections.

靶标信息

Tau is a neuronal microtubule-associated protein found predominantly on axons. The function of Tau is to promote tubulin polymerization and stabilize microtubules. The C-terminus binds axonal microtubules while the N- terminus binds neural plasma membrane components, suggesting that tau functions as a linker protein between both. Axonal polarity is predetermined by TAU/MAPT localization (in the neuronal cell) in the domain of the cell body defined by the centrosome. The short isoforms allow plasticity of the cytoskeleton while the longer isoforms may preferentially play a role in its stabilization. In its hyper-phosphorylated form, Tau is the major component of paired helical filaments (PHF), the building block of neurofibrillary lesions in Alzheimer's diseases (AD) brain. Hyper-phosphorylation impairs the microtubule binding function of Tau, resulting in the destabilization of microtubules in AD brains, ultimately leading to the degeneration of the affected neurons. Numerous serine/threonine kinases phosphorylate Tau, including GSK-3beta, protein kinase A (PKA), cyclin-dependent kinase 5 (cdk5) and casein kinase II. Hyper-phosphorylated Tau is found in neurofibrillary lesions in a range and other central nervous system disorders such as Pick's disease, frontotemporal dementia, cortico-basal degeneration and progressive supranuclear palsy.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: FLJ31424; FTDP17; G protein beta1/gamma2 subunit-interacting factor 1; map tau; MGC138549; Microtubule-associated protein tau; microtubule-associated protein tau, isoform 4; microtubules; Neurofibrillary tangle protein; neurofibrillary tangles; Neuronal Marker; Paired helical filament-tau; PHF-tau; PHFtau; protein phosphatase 1, regulatory subunit 103; Tau microtubule-associated protein; Tau-4; Tau5

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基因别名: AI413597; AW045860; DDPAC; FTDP-17; MAPT; MAPTL; MSTD; Mtapt; MTBT1; MTBT2; PPND; PPP1R103; pTau; RNPTAU; TAU

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UniProt ID: (Bovine) P29172, (Human) P10636, (Mouse) P10637