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Agrisera
Thioredoxin-like protein HCF164, chloroplastic Polyclonal Antibody
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Thioredoxin-like protein HCF164, chloroplastic Antibody (AS16-4097) in WB
Arabidopsis thaliana Col 0 thylakoids were extracted in isolation buffer (330 mm Sucrose, 25 mm HEPES-KOH, p H 7.4,10 mm Mg Cl 2, and 10 mm Na F) and denatured with LaemmLi buffer at 65 C for 10 min. Proteins were separated on 12% SDS-PAGE and blotted 1 h on a PVDF membrane using semi-dry transfer. Blot was blocked with 8% milk for 1 h at RT. Blot was incubated in the primary antibody at a dilution of 1:3,0... View More
产品信息
AS16-4097
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana HCF164 protein sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Lyophilized antibody can be stored at -20°C for up to 3 years. Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
HCF164 encodes a nuclear-encoded chloroplast protein that localizes to the thylakoid membrane with its functional redox domain exposed to the thylakoid lumen, consistent with a role in lumenal thiol-disulfide chemistry during photosynthetic complex maturation. The protein is reported to be ~261 amino acids and organized into an N-terminal hydrophilic region bearing features consistent with a chloroplast targeting/transit sequence, followed by a single hydrophobic transmembrane segment that anchors it in the thylakoid, and a C-terminal thioredoxin-like domain containing the canonical active-site WCxxC motif required for disulfide reductase activity. Functionally, genetic and biochemical data indicate HCF164 is indispensable for post-translational biogenesis/assembly of the cytochrome b6f complex, and it participates in transferring reducing equivalents into the lumen: stromal m-type thioredoxin can provide reducing power (indirectly across the membrane) to maintain HCF164 in a reduced state, enabling HCF164 to reduce lumen-facing target proteins, including components linked to cytochrome b6f accumulation and additional thylakoid substrates such as the PSI-N subunit, thereby supporting proper assembly and/or maintenance of photosynthetic electron transport machinery.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AP22.37; AP22_37; AT4G37200; C7A10.160; HIGH CHLOROPHYLL FLUORESCENCE 164
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