Invitrogen

Thrombospondin 1 Polyclonal Antibody

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问题与答案

问题与答案

问题与答案

抗体检测数据 (1)

Thrombospondin 1 Antibody in Western Blot (WB)

Thrombospondin 1 Antibody in Western Blot (WB)

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Thrombospondin 1 Antibody (PA5-95445) in WB

Western blot analysis of Thrombospondin in, Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: HELA whole Cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was in... View More

Thrombospondin 1 Antibody in Western Blot (WB)

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Thrombospondin 1 Antibody in Western Blot (WB) — Western blot analysis of Thrombospondin in, Lane 1: rat liver tissue lysates, Lane 2: mouse liver tissue lysates, Lane 3: HELA whole Cell lysates. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 µg of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. The membrane was blocked with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with Thrombospondin 1 Polyclonal Antibody (Product # PA5-95445) at 0.5 μg/mL overnight at 4°C, then washed with TBS-0. 1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit. A specific band was detected for Thrombospondin at approximately 165KD., 180KD. The expected band size for Thrombospondin is at 130KD.

Immunogen image

Thrombospondin 1 Polyclonal Antibody

Immunogen Image Thrombospondin 1 Polyclonal Antibody

产品信息

PA5-95445

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

-

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

-

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human Thrombospondin recombinant protein (Position: D27-H112).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

Human Thrombospondin shares 81.4% amino acid (aa) sequence identity with mouse Thrombospondin.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: rat liver tissue, mouse liver tissue, HELA whole cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Thrombospondin is a protein from platelet a-granules. It is made up of three identical subunits bound by interchain disulfides. It is secreted at sites of platelet activation and aggregation and is involved in the processes of chemotaxis, adhesion, proliferation and differentiation of leukocytes, fibroblasts, smooth muscle and endothelial cells.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Glycoprotein G; Thrombospondin-1; thrombospondin-1p180; thrombospondin-p50; ThrombospondinTHBS1

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基因别名: tbsp1; THBS; THBS-1; THBS1; TSP; TSP-1; TSP1

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UniProt ID: (Human) P07996

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Entrez Gene ID: (Human) 7057, (Rat) 445442, (Mouse) 21825

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phosphatidylserine binding glycoprotein binding fibronectin binding integrin binding calcium ion binding protein binding heparin binding fibroblast growth factor binding low-density lipoprotein particle binding identical protein binding laminin binding proteoglycan binding transforming growth factor beta binding fibrinogen binding collagen V binding extracellular matrix binding

activation of MAPK activity response to hypoxia negative regulation of endothelial cell proliferation negative regulation of cell-matrix adhesion sprouting angiogenesis chronic inflammatory response platelet degranulation negative regulation of antigen processing and presentation of peptide or polysaccharide antigen via MHC class II negative regulation of dendritic cell antigen processing and presentation inflammatory response immune response response to unfolded protein cell cycle arrest cell adhesion response to glucose positive regulation of endothelial cell migration negative regulation of endothelial cell migration negative regulation of plasma membrane long-chain fatty acid transport negative regulation of nitric oxide mediated signal transduction negative regulation of cGMP-mediated signaling negative regulation of plasminogen activation positive regulation of macrophage chemotaxis positive regulation of fibroblast migration cell migration negative regulation of angiogenesis peptide cross-linking positive regulation of blood coagulation extracellular matrix organization positive regulation of cell migration positive regulation of transforming growth factor beta receptor signaling pathway regulation of cGMP metabolic process response to magnesium ion response to progesterone negative regulation of interleukin-12 production positive regulation of transforming growth factor beta1 production cellular response to heat response to endoplasmic reticulum stress protein O-linked fucosylation negative regulation of fibroblast growth factor receptor signaling pathway positive regulation of phosphorylation response to drug positive regulation of tumor necrosis factor biosynthetic process positive regulation of macrophage activation negative regulation of apoptotic process negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of blood vessel endothelial cell migration negative regulation of blood vessel endothelial cell migration engulfment of apoptotic cell positive regulation of translation positive regulation of angiogenesis behavioral response to pain positive regulation of chemotaxis response to calcium ion negative regulation of focal adhesion assembly positive regulation of protein kinase B signaling negative regulation of fibrinolysis positive regulation of extrinsic apoptotic signaling pathway via death domain receptors positive regulation of endothelial cell apoptotic process positive regulation of reactive oxygen species metabolic process negative regulation of endothelial cell chemotaxis negative regulation of extrinsic apoptotic signaling pathway outflow tract morphogenesis endocardial cushion development growth plate cartilage development response to mechanical stimulus positive regulation of cell-substrate adhesion response to testosterone blood vessel morphogenesis cellular response to tumor necrosis factor cellular response to growth factor stimulus positive regulation of transforming growth factor beta production cellular response to nitric oxide