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Agrisera
Toc75/Protein TOC75-3, chloroplastic, POTRA domain 3 Polyclonal Antibody
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Toc75/Protein TOC75-3, chloroplastic, POTRA domain 3 Antibody (AS08-351) in WB
Samples of crude Arabidopsis thaliana chloroplast preparation from 250 xg pellet (1), unknown sample (2), Arabidopsis thaliana 100 k Da membrane preparation (3), Prestained marker 80 k Da band (NEB Broad Range) (4), Physcomitrella patens ‘broken’ chloroplasts from above 40% on Percoll gradient (5), unknown sample (6), Physcomitrella patens intact chloroplasts from above 80% on Percoll gradient (7) were blot... View More
产品信息
AS08-351
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
Pisum sativum TOC75.
偶联物
形式
Lyophilized
保存液
whole serum
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana, Nicotiana benthamiana, Nicotiana tabacum, Physcomitrium patens, Pisum sativum, Spinacia oleracea, Thellungiella salsuginea, some cross-reactivity was observed for cyanobacteria including: Synechocystis, Synechococcus and Thermosynechococcus sp.
靶标信息
Toc75 is an essential Omp85-family beta-barrel protein embedded in the chloroplast outer envelope membrane, where it constitutes the core protein-conducting pore of the TOC (translocon at the outer chloroplast membrane) import machinery for nuclear-encoded preproteins. It is synthesized as a precursor that is sorted to the outer envelope, and mature Toc75 is organized as an N-terminal soluble region exposed to the intermembrane space containing three polypeptide transport-associated (POTRA) domains, followed by a C-terminal membrane-integrated beta-barrel predicted to comprise 16 transmembrane beta strands that form the translocation channel. Functionally, Toc75 cooperates with TOC receptor GTPases (e.g., Toc34 and Toc159-family receptors) to receive cytosolic preproteins bearing chloroplast transit peptides and to mediate their passage across the outer membrane en route to downstream TIC components at the inner envelope; biophysical and genetic data support its role as a selective, gated pore that can interact with transit peptides and is required for efficient chloroplast protein import and organelle biogenesis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 46082; AT3G46740; T6H20.230; TOC75; TOC75-3; TOC75-III
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