Invitrogen

Transferrin Receptor Polyclonal Antibody

Name: Transferrin Receptor Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-95212

查看其他128个Transferrin Receptor (CD71)抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (8)

Transferrin Receptor Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 8

Transferrin Receptor Antibody (PA5-95212) in ICC/IF

Immunocytochemistry analysis of TFRC using anti-TFRC antibody (Product # PA5-95212). TFRC was detected in a section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and then incubated with 2μg/mL rabbit anti-TFRC antibody (Product # PA5-95212) overnight at 4°C. Cy3 Conjugated Goat Anti-Rabbit IgG was used... View More

Transferrin Receptor Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Transferrin Receptor Antibody in Immunohistochemistry (Frozen) (IHC (F))

Transferrin Receptor Antibody in Western Blot (WB)

Transferrin Receptor Antibody in Flow Cytometry (Flow)

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Immunogen image

Immunogen Image Transferrin Receptor Polyclonal Antibody

产品信息

PA5-95212

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫组化（冰冻） (IHC (F))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human TFRC recombinant protein (Position: M1-N198).

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 5mg BSA

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2807016

产品详细信息

Human TFRC shares 72% and 70% amino acid (aa) sequences identity with mouse and rat TFRC, respectively.

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human Hela whole cell, human K562 whole cell, human HT1080 whole cell, rat thymus tissue, rat RAW2647 whole cell, mouse SP2/0 whole cell. IHC: human placenta tissue IHC-F: human placenta tissue. ICC/IF: A431 cell. Flow: SiHa cell, U87 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Cellular uptake of iron occurs via receptor-mediated endocytosis of ligand-occupied transferrin receptor into specialized endosomes. Endosomal acidification leads to iron release. The apotransferrin-receptor complex is then recycled to the cell surface with a return to neutral pH and the concomitant loss of affinity of apotransferrin for its receptor. Transferrin receptor is necessary for development of erythrocytes and the nervous system. Serum transferrin receptor (sTfR) is used as a means of detecting erythropoietin (EPO) misuse by athletes and as a diagnostic test for anemias resulting from a number of conditions including rheumatoid arthritis, pregnancy, irritable bowel syndrome and in HIV patients.

仅用于科研。不用于诊断过程。未经明确授权不得转售。