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Agrisera
TrxM1/M2/Thioredoxin M1/M2 (chloroplastic) Polyclonal Antibody
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TrxM1/M2/Thioredoxin M1/M2 (chloroplastic) Antibody (AS14-2809) in WB
7.5 and 15 µg of soluble protein extract from WT-Col-0 Arabidopsis thaliana extracted in a buffer containing 50 m M HEPES, 5 m M Na Cl and 10 m M Mg Cl 2, separated on 12% SDS-PAGE and blotted 1 h to PVDF using semi-dry transfer. Blots were blocked with 4% milk for 1 h at room temperature (RT) with agitation. Blot was incubated in the primary antibody at a dilution of 1:1,000 overnight in 4°C with agitation... View More
产品信息
AS14-2809
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide, derived from Arabidopsis thaliana TrxM1 and TrxM2.
偶联物
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
Thioredoxin m1 and thioredoxin m2 are nuclear-encoded, chloroplast-targeted m-type thioredoxins that accumulate predominantly in the chloroplast stroma, where they participate in the ferredoxin-dependent thiol redox network that links light-driven electron flow to reversible control of protein disulfide status. After import, each protein is a small soluble oxidoreductase built around the conserved thioredoxin fold and a catalytic Cys-x-x-Cys active-site motif that cycles between reduced dithiols and an intramolecular disulfide, enabling them to reduce regulatory disulfide bonds in client proteins. Functionally, m-type thioredoxins act as major redox regulators of photosynthetic metabolism in vivo, contributing to light activation of Calvin-Benson cycle enzymes (including fructose-1,6-bisphosphatase and sedoheptulose-1,7-bisphosphatase) and thereby supporting CO2 assimilation and growth; genetic depletion also reveals broader roles in chloroplast performance, including support of photosystem II biogenesis and stability through redox-dependent interactions with assembly intermediates and core subunits.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATHM1; ATHM2; ATM1; ATM2; THM1; THM2; TRX-M1; TRX-M2
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