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Invitrogen
TurboLuc luciferase Polyclonal Antibody
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TurboLuc luciferase Antibody (PA1-178) in WB
Western blot analysis of TurboLuc luciferase was performed by loading the indicated amounts of recombinant TurboLuc whole cell lysates, and 10 µL of PageRuler Plus Prestained Protein Ladder (Product # 26619) onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to a PVDF membrane using the G2 Fast Blotter (Product # 62288), and blocked with StartingBlock T20 (TBS) Blocking Buffer (Product # 37543) for 1 hour at room temperature. TurboLuc luciferase was detected at 16 kDa using a TurboLuc luciferase polyclonal antibody (Produc... View More
产品信息
PA1-178
产品规格
种属反应
Luciferase
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Purified recombinant TurboLuc luciferase expressed in E. coli.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein G
保存液
PBS with 1mg/mL BSA, 30% glycerol
内含物
0.05% sodium azide
保存条件
-20°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Western blot analysis of PA1-178 detects an ~16-18 kDa protein in Tluc expressing cells.
PA1-178 can be used for activity-independent verification of Tluc expression in qIP assays.
靶标信息
TurboLuc™ (Tluc) is a novel intracellular luciferase derived from the marine copepod Metridia luciferase family. The Tluc gene was developed using directed mutagenesis for optimal performance and codon optimized for mammalian cell expression. Tluc is an ATP-independent enzyme that utilizes the substrate coelenterazine to produce intense bioluminescence light. Tluc is smaller and brighter than other commonly used luciferases, including firefly and Renilla. The intense bioluminescence greatly enhances sensitivity of Tluc luciferase-based assays, enabling detection of very minute amounts of luciferase (signal) in very sensitive assays such as protein interaction qIP assays (Prod # 82032, 82033, 82035, 82036).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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