Invitrogen

Twist1/Twist2 Polyclonal Antibody

View all (2) Twist1/Twist2 antibodies

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Datasheet
Protocols
Questions & Answers
Tech Support

Product Details

PA5-78211

Applications
Tested Dilution
Publications

Western Blot (WB)

1:500-1:10,000
-

Immunohistochemistry (Paraffin) (IHC (P))

1:100-1:1,000
-
Product Specifications

Species Reactivity

Fruit fly, Human, Mouse, Rat

Host/Isotype

Rabbit / IgG

Class

Polyclonal

Type

Antibody

Immunogen

Recombinant protein encompassing a sequence within the center region of human Twist1/2. The exact sequence is proprietary.

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.67 mg/mL

Purification

Antigen affinity chromatography

Storage buffer

PBS, pH 7, with 20% glycerol

Contains

0.025% ProClin 300

Storage conditions

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2736761

Product Specific Information

Positive Control: Raji, THP-1, HL-60, TWIST1 transfected 293T lysates, human TWIST1-transfected 293T, Jurkat, OECM1, CL1-5, Toledo, SU-DHL-5, HT, DB, RL95-2, MDA-MB-231

Predicted Reactivity: Japanese Medaka (95%), Xenopus laevis (97%), Chicken (100%), Chimpanzee (100%), Bovine (100%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

Target Information

Twist1/Twist2 encodes a basic helix-loop-helix (bHLH) transcription factor that plays an important role in embryonic development. The encoded protein forms both homodimers and heterodimers that bind to DNA E box sequences and regulate the transcription of genes involved in cranial suture closure during skull development. This protein may also regulate neural tube closure, limb development and brown fat metabolism. Twist1/Twist2 is hypermethylated and overexpressed in multiple human cancers, and the encoded protein promotes tumor cell invasion and metastasis. Mutations in this gene cause Saethre-Chotzen syndrome in human patients, which is characterized by craniosynostosis, ptosis and hypertelorism.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Bioinformatics

Protein Aliases: B-HLH DNA binding protein; bHLHa38; bHLHa39; charlie chaplin; Class A basic helix-loop-helix protein 38; Class A basic helix-loop-helix protein 39; dermis expressed 1; Dermis-expressed protein 1; Dermo-1; dermo-1 protein; H-twist; M-twist; MGC117334; Pluridigite; polydactyly EMS; Skam10Jus; twist basic helix-loop-helix transcription factor 1; twist basic helix-loop-helix transcription factor 2; twist gene homolog 1; twist homolog 1; twist homolog 2; TWIST homolog of drosophila; twist-related bHLH protein Dermo1; Twist-related protein 1; Twist-related protein 2

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Gene Aliases: AA960487; ACS3; AMS; BBRSAY; BHLHA38; BHLHA39; BPES2; BPES3; CRS; CRS1; CSO; DERMO1; FFDD3; M-Twist; Pde; pdt; SCS; SETLSS; Ska10; Ska; TWIST; TWIST1; TWIST2

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UniProt ID: (Human) Q15672, (Human) Q8WVJ9, (Mouse) P26687, (Mouse) Q9D030, (Rat) P97831

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Entrez Gene ID: (Human) 7291, (Human) 117581, (Mouse) 22160, (Rat) 85489, (Mouse) 13345, (Rat) 59327

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Function(s)
RNA polymerase II transcription factor activity, sequence-specific DNA binding protein binding transcription factor binding protein domain specific binding protein homodimerization activity bHLH transcription factor binding protein heterodimerization activity E-box binding DNA binding protein dimerization activity transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding molecular_function transcription factor activity, RNA polymerase II transcription factor binding chromatin binding basic helix-loop-helix transcription factor
Process(es)
negative regulation of transcription from RNA polymerase II promoter ossification osteoblast differentiation in utero embryonic development neuron migration neural tube closure aortic valve morphogenesis mitral valve morphogenesis endocardial cushion morphogenesis cardiac neural crest cell migration involved in outflow tract morphogenesis transcription, DNA-templated muscle organ development positive regulation of gene expression positive regulation of epithelial to mesenchymal transition negative regulation of phosphatidylinositol 3-kinase signaling regulation of bone mineralization positive regulation of fatty acid beta-oxidation negative regulation of tumor necrosis factor production positive regulation of tumor necrosis factor production negative regulation of histone phosphorylation negative regulation of histone acetylation embryonic forelimb morphogenesis embryonic hindlimb morphogenesis negative regulation of peroxisome proliferator activated receptor signaling pathway outer ear morphogenesis odontogenesis embryonic digit morphogenesis negative regulation of apoptotic process negative regulation of sequence-specific DNA binding transcription factor activity negative regulation of DNA damage response, signal transduction by p53 class mediator negative regulation of osteoblast differentiation positive regulation of angiogenesis negative regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter rhythmic process negative regulation of skeletal muscle tissue development embryonic cranial skeleton morphogenesis positive regulation of epithelial cell proliferation palate development cranial suture morphogenesis embryonic camera-type eye formation eyelid development in camera-type eye cellular response to growth factor stimulus cellular response to hypoxia positive regulation of monocyte chemotactic protein-1 production positive regulation of DNA-templated transcription, initiation positive regulation of cell motility negative regulation of oxidative phosphorylation uncoupler activity positive regulation of transcription regulatory region DNA binding negative regulation of cellular senescence positive regulation of interleukin-6 secretion negative regulation of double-strand break repair cell proliferation involved in heart valve development positive regulation of endocardial cushion to mesenchymal transition involved in heart valve formation multicellular organism development cell differentiation regulation of transcription, DNA-templated transcription from RNA polymerase II promoter embryonic limb morphogenesis hindlimb morphogenesis negative regulation of molecular function negative regulation of cell differentiation negative regulation of striated muscle tissue development embryonic skeletal system morphogenesis cardiac neural crest cell development involved in outflow tract morphogenesis bone development regulation of transcription from RNA polymerase II promoter negative regulation of cell proliferation positive regulation of keratinocyte proliferation negative regulation of DNA binding negative regulation of myeloid cell differentiation face morphogenesis cornea development in camera-type eye
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