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U1 snRNP protein A/U1 small nuclear ribonucleoprotein A Polyclonal Antibody
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U1 snRNP protein A/U1 small nuclear ribonucleoprotein A Antibody (AS14-2777) in WB
Total protein from rosette leaves> 30 µg of Arabidopsis thaliana whole leaf extract (wild-type) (1), 30 µg of Arabidopsis thaliana whole leaf extract (u 1 a) (2), extracted with buffer (100 m M Tris, 10% glycerol, 5 m M EDTA, 5 m M EGTA, 0.15 M Na Cl, 0.75% Triton X 100,0.05% SDS, 1 m M DTT) and denatured with 0.06 M Tris-HCl, 5% glycerol, 2% SDS, 4% β-mercaptoethanol, 0.25% bromophenol blue at 95 C for 5 m... View More
产品信息
AS14-2777
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide, derived from Arabidopsis thaliana U1 snRNP.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
U1A (encoded by SNRPA) is a predominantly nuclear, spliceosome-associated RNA-binding protein enriched in nucleoplasm/nuclear speckles, where it forms part of the U1 small nuclear ribonucleoprotein particle that recognizes 5-prime splice sites during pre-mRNA splicing. The protein is built around two RNA recognition motif (RRM) domains, with an N-terminal RRM that binds with high affinity and sequence specificity to stem-loop II of U1 snRNA, while the C-terminal RRM is less clearly assigned to RNA binding and is thought to support additional protein-protein interactions within RNA-processing assemblies; regulatory elements including a nuclear localization signal contribute to its nuclear accumulation. Functionally, U1A stabilizes and positions U1 snRNP on nascent transcripts to promote intron removal and has a well-defined autoregulation mechanism in which excess U1A binds a conserved element in its own 3-prime untranslated region and inhibits polyadenylation of its pre-mRNA via a dimerization-dependent process, linking splicing-factor availability to 3-prime end formation and transcript output.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Mud1; U1-A; U1A
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