Invitrogen

UBE2D1/2/3/4 Polyclonal Antibody

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说明书
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问题与答案
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说明书
实验方案
问题与答案
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说明书
实验方案
问题与答案
技术支持
UBE2D1/2/3/4 Polyclonal Antibody

产品信息

PA5-143926

应用
建议稀释比
已发表文章

免疫印迹 (WB)

0.25-0.5 µg/mL
-

免疫组化（石蜡） (IHC (P))

2-5 µg/mL
-

免疫细胞化学 (ICC/IF)

5 µg/mL
-

流式细胞分析 (Flow)

1-3 µg/10x^6 cells
-

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL
-
产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human UBE2D1/2/3/4 recombinant protein (Position: D116-M147).
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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.005mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_3075140

产品详细信息

Adding 0.2 mL of distilled water will yield a concentration of 500 µg/mL.

Positive Control - WB: rat heart tissue, rat lung tissue, rat liver tissue, human HEK293 whole cell, human Jurkat whole cell, human CACO-2 whole cell. IHC: mouse lung lymph node tissue, human testicular cancer tissue, human bladder cancer tissue, rat lung tissue. ICC/IF: A431 cell. Flow: A431 cell, RAW2647 cell, RH35 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

Ubiquitination is a post-translational modification pathway or is part of the specific protein-degradation pathway by the 26S proteasome. Ubiquitination of a target protein involves multistep enzymatic reaction catalyzed by a cascade of enzymes including Ub-activating enzymes (E1s), Ub-conjugating enzymes (E2s) and Ub ligases (E3s) (PMID: 23542885). UBE2D family, which has 4 members named as UBE2D1/2/3/4, is an E2 ubiquitin-conjugating enzyme family in the ubiquitin-proteasome system. This antibody can recognize all the four members of UBE2D due to the high homology.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: (E3-independent) E2 ubiquitin-conjugating enzyme D1; (E3-independent) E2 ubiquitin-conjugating enzyme D2; (E3-independent) E2 ubiquitin-conjugating enzyme D3; E2 ubiquitin-conjugating enzyme D1; E2 ubiquitin-conjugating enzyme D2; E2 ubiquitin-conjugating enzyme D2B; E2 ubiquitin-conjugating enzyme D3; E2 ubiquitin-conjugating enzyme D4; E2(17)KB 1; E2(17)KB 2; E2(17)KB 2B; E2(17)KB 3; HBUCE1; p53-regulated ubiquitin-conjugating enzyme 1; PAPase; Phosphoarginine phosphatase; PRO2116; SFT; Stimulator of Fe transport; ubc2e ubiquitin conjugating enzyme; UBC4/5 homolog; UbcH5; UBE2D1/UBE2D3/UBE2D3/UBE2D4; Ubiquitin carrier protein D1; Ubiquitin carrier protein D2; Ubiquitin carrier protein D2B; Ubiquitin carrier protein D3; Ubiquitin carrier protein D4; ubiquitin conjugating enzyme E2D 1; ubiquitin conjugating enzyme E2D 2; ubiquitin conjugating enzyme E2D 3; ubiquitin conjugating enzyme E2D 4 (putative); Ubiquitin-conjugating enzyme E2 D1; Ubiquitin-conjugating enzyme E2 D2; Ubiquitin-conjugating enzyme E2 D2B; Ubiquitin-conjugating enzyme E2 D3; Ubiquitin-conjugating enzyme E2 D4; Ubiquitin-conjugating enzyme E2(17)KB 1; Ubiquitin-conjugating enzyme E2(17)KB 2; Ubiquitin-conjugating enzyme E2(17)KB 2B; Ubiquitin-conjugating enzyme E2(17)KB 3; Ubiquitin-conjugating enzyme E2-17 kDa 1; Ubiquitin-conjugating enzyme E2-17 kDa 2; Ubiquitin-conjugating enzyme E2-17 kDa 2B; Ubiquitin-conjugating enzyme E2-17 kDa 3; ubiquitin-conjugating enzyme E2D 1 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2D 1, UBC4/5 homolog; ubiquitin-conjugating enzyme E2D 2; ubiquitin-conjugating enzyme E2D 2 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 2 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2D 3 (homologous to yeast UBC4/5); ubiquitin-conjugating enzyme E2D 3 (UBC4/5 homolog, yeast); ubiquitin-conjugating enzyme E2D 4 (putative); ubiquitin-conjugating enzyme HBUCE1; Ubiquitin-protein ligase D1; Ubiquitin-protein ligase D2; Ubiquitin-protein ligase D2B; Ubiquitin-protein ligase D3; Ubiquitin-protein ligase D4

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基因别名: 1100001F19Rik; 9430029A22Rik; AA414951; E2(17)KB1; E2(17)KB2; E2(17)KB3; E217kB; HBUCE1; PUBC1; RGD69425; SFT; Ubc2e; UBC4; UBC4/5; UBC5A; UBC5B; UBC5C; UBCH4; UBCH5; UBCH5A; UBCH5B; UBCH5C; UBCH5D; UBE2D1; UBE2D2; Ube2d2b; UBE2D3; UBE2D4

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UniProt ID: (Human) P51668, (Human) P62837, (Human) P61077, (Human) Q9Y2X8, (Mouse) P61080, (Rat) D3ZDK2, (Mouse) P61079, (Rat) P61078, (Rat) P70711

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Entrez Gene ID: (Human) 7321, (Human) 7322, (Human) 7323, (Human) 51619, (Mouse) 216080, (Rat) 361831, (Rat) 641452, (Mouse) 66105, (Rat) 81920, (Rat) 79435

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功能
ubiquitin-protein transferase activity protein binding ATP binding ubiquitin conjugating enzyme activity nucleotide binding ligase activity molecular_function ubiquitin-protein ligase
参与通路
negative regulation of transcription from RNA polymerase II promoter protein polyubiquitination stimulatory C-type lectin receptor signaling pathway ubiquitin-dependent protein catabolic process BMP signaling pathway anaphase-promoting complex-dependent catabolic process positive regulation of protein ubiquitination TRIF-dependent toll-like receptor signaling pathway Fc-epsilon receptor signaling pathway protein ubiquitination involved in ubiquitin-dependent protein catabolic process proteasome-mediated ubiquitin-dependent protein catabolic process T cell receptor signaling pathway negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle regulation of transcription from RNA polymerase II promoter in response to hypoxia protein K48-linked ubiquitination cellular protein modification process protein ubiquitination protein autoubiquitination DNA repair protein monoubiquitination apoptotic process protein K11-linked ubiquitination positive regulation of protein targeting to mitochondrion protein K29-linked ubiquitination protein K27-linked ubiquitination protein K63-linked ubiquitination protein K6-linked ubiquitination metabolic process cellular response to DNA damage stimulus biological_process
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