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Bethyl Laboratories
UBF Polyclonal Antibody
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UBF Antibody (A301-859A) in IP
Detection of human UBF by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-UBF antibody (Product # A301-859A) used for IP at 3 µg/mg lysate. UBF was also immunoprecipitated by rabbit anti-UBF antibody BL7375, which recognizes an upstream epitope. For blotting immunoprecipitated UBF, A301-859A was used at 1 µg/mL. Detection: Chemiluminescence with an exposure time of 15 seconds.
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
A301-859A
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Region between residue 546 and 596 of Human upstream binding transcription factor, RNA polymerase I.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1 mg/mL
规格
100 µg
纯化类型
Antigen affinity chromatography
保存液
tris citrate/phosphate, pH 7-8
内含物
0.09% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
The recommended shelf life for this product is 1 year from date of receipt.
Based on 100% sequence identity, this antibody is predicted to react with Mouse, Rat and X. laevis
靶标信息
UBF-1 encodes a member of the HMG-box DNA-binding protein family. The encoded protein plays a critical role in ribosomal RNA transcription as a key component of the pre-initiation complex, mediating the recruitment of RNA polymerase I to rDNA promoter regions. The encoded protein may also play important roles in chromatin remodeling and pre-rRNA processing, and its activity is regulated by both phosphorylation and acetylation. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. Pseudogenes of this gene are located on the short arm of chromosomes 3, 11 and X and the long arm of chromosome 11.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Autoantigen NOR-90; NOR90; Nucleolar transcription factor 1; UBF-1; Upstream-binding factor 1
基因别名: UBF; UBF1; UBTF
Entrez Gene ID: (Human) 7343
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
