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Rockland
UBP43 Polyclonal Antibody
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UBP43 Antibody (600-401-476) in WB
Western blot using Rocklands Affinity Purified anti-UBP43 antibody shows detection of a band ~43 kDa band (arrowhead) believed to be to UBP43 in lysates from HeLa nuclear extracts (lane 1) and whole cell lysates from HeLa (lane 2), A431 (lane 3), Jurkat (Lane 4) and HEK293 (lane 5). Approximately 18 µg of each lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-UBP43 antibody. Signal was detected using standard techniques. The strong band between the 37kD and 50kD markers is e... View More
产品信息
600-401-476
应用
建议稀释比
已发表文章
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 25-50 of human UBP43 protein.
偶联物
形式
Liquid
浓度
0.9 mg/mL
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
This affinity-purified antibody is directed against human UBP43 protein. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee and orangutan based on 100% homology for the immunogen sequence. The core 7 amino acid sequence QPRERPR of the 10 amino acid immunogen sequence may be reactive with UBP43 from mouse and dog. This core sequence also shows partial homology with proteins other than UBP43. Cross reactivity with UBP43 homologues from other sources has not been determined.
靶标信息
USP18, a member of the deubiquitinating protease family of enzymes, removes ubiquitin adducts from a broad range of protein substrates.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 43 kDa ISG15-specific protease; hUBP43; ISG15-specific-processing protease; ubiquitin specific protease 18; Ubl carboxyl-terminal hydrolase 18; Ubl thioesterase 18; ubl thiolesterase 18
基因别名: ISG43; UBP43; USP18
UniProt ID: (Human) Q9UMW8
Entrez Gene ID: (Human) 11274
