Invitrogen

UGT2B10 Monoclonal Antibody (1B5)

Name: UGT2B10 Monoclonal Antibody (1B5)
Brand: Invitrogen
SKU: MA5-24546

查看其他4个UGT2B10抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Immunogen image

Immunogen Image UGT2B10 Monoclonal Antibody (1B5)

产品信息

MA5-24546

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-5 µg/mL

酶联免疫吸附实验 (ELISA)

Assay-Dependent

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

1B5

抗原

UGT2B10 (NP_001066, 62 a.a. ~ 159 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

纯化类型

Protein A

保存液

PBS, pH 7.4

内含物

no preservative

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2633518

产品详细信息

Peptide Sequence: LFDPNDSSTL KLEVYPTSLT KTEFENIIMQ LVKRLSEIQK DTFWLPFSQE QEILWAINDI IRNFCKDVVS NKKLMKKLQE SRFDIVFADA YLPCGELL

靶标信息

DNA containing double-strand breaks (DSB) created by the CRISPR/Cas9 system can be repaired by either the non-homologous end-joining (NHEJ) or the homology-directed repair (HDR) pathway. The NHEJ repair pathway introduces non-specific insertions or deletions at the cleavage site, whereas the HDR pathway allows for precise gene editing at the DSB site. Target-specific HDR Plasmids provide a DNA repair template for a DSB and, when co-transfected with CRISPR/Cas9 KO Plasmids, enable the insertion of specific selection markers where Cas9-induced DNA cleavage has occurred. The HDR plasmid can incorporate a Red Fluorescent Protein (RFP) gene to visually confirm transfection and an antibiotic resistance gene (puromycin) for selection of cells containing a successful CRISPR/Cas9 doublestrand break. The puromycin resistance and RFP encoding genes are flanked by two LoxP sites that are recognized by the Cre Vector, which can be used to later remove these selection markers from the genomic DNA.

仅用于科研。不用于诊断过程。未经明确授权不得转售。