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Agrisera
UVR3/Protein UV repair defective 3 Polyclonal Antibody
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UVR3/Protein UV repair defective 3 Antibody (AS16-4069) in WB
Samples: 1,2 - Cell lysate from Arabidopsis thaliana leaves, overexpressing UVR 3 fused to GFP. 3,4 - UVR 3-GFP immunoprecipitated using GFP-Trap Agarose beads (Chromotec) from Arabidopsis thaliana leaves overexpressing UVR 3 fused to GFP Cell lysate from E. coli expressing recombinant protein UVR 3 with his-tag. 5,6 – before induction 7,8 – after induction, and production of protein Samples were separated ... View More
产品信息
AS16-4069
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana UVR3 protein sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana UVR3-GFP
靶标信息
UVR3 encodes a DNA photolyase family enzyme that localizes predominantly to the nucleus and is also detected in chloroplasts and mitochondria, consistent with a role in protecting multiple plant genomes from ultraviolet damage. The UVR3 protein is a flavoprotein photolyase/cryptochrome-like polypeptide that contains the conserved N-terminal photolyase-related region and a C-terminal FAD-binding domain typical of 6-4 photoproduct photolyases, enabling binding of a catalytic flavin cofactor and light-driven chemistry. Functionally, UVR3 is required for photorepair (photoreactivation) of UV-induced pyrimidine-pyrimidone (6-4) photoproducts in vivo; loss-of-function mutants show defective 6-4 lesion reversal, and heterologous expression demonstrates 6-4 photolyase activity, supporting a direct enzymatic role in blue/UVA light-dependent restoration of normal DNA structure.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: 6-4 PHOTOLYASE; AT3G15620; AtUVR3; MSJ11.2; UV REPAIR DEFECTIVE 3
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