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Ubiquitin Monoclonal Antibody (FK2), FITC

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产品信息

MA5-45329

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:10,000
-

免疫组化 (IHC)

Assay-dependent
-

免疫细胞化学 (ICC/IF)

Assay-dependent
-

酶联免疫吸附实验 (ELISA)

1:1,000
-

免疫沉淀 (IP)

Assay-dependent
-
产品规格

种属反应

All, Human

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

FK2

抗原

Ubiquitin conjugated lysozyme

偶联物

FITC FITC FITC

激发/发射光谱

498/517 nm 查看光谱 spectra

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

9.09mM sodium bicarbonate/PBS, pH 7.4, with 640.91mM DMSO, 136.36mM ethanolamine

内含物

no preservative

保存条件

4°C, store in dark

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2931783

产品详细信息

A 1:5000 dilution of MA5-45329 was sufficient for detection of ubiquitin conjugates in 2.5 µg of HeLa cell lysate by electrochemiluminescence analysis using goat anti-mouse IgG:HRP as the secondary antibody.|Detects ubiquitinated proteins and ubiquitin chains. Does not detect free ubiquitin.

靶标信息

Ubiquitin, a highly conserved protein that has a major role in targeting cellular proteins for degradation by the 26S proteosome, is synthesized as a precursor protein consisting of either polyubiquitin chains or a single ubiquitin fused to an unrelated protein. This gene encodes a fusion protein consisting of ubiquitin at the N terminus and ribosomal protein S27a at the C terminus. When expressed in yeast, the protein is post-translationally processed, generating free ubiquitin monomer and ribosomal protein S27a. Ribosomal protein S27a is a component of the 40S subunit of the ribosome and belongs to the S27AE family of ribosomal proteins. It contains C4-type zinc finger domains and is located in the cytoplasm. Pseudogenes derived from this gene are present in the genome. As with ribosomal protein S27a, ribosomal protein L40 is also synthesized as a fusion protein with ubiquitin; similarly, ribosomal protein S30 is synthesized as a fusion protein with the ubiquitin-like protein fubi. Multiple alternatively spliced transcript variants that encode the same proteins have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 40S ribosomal protein S27-like; 40S ribosomal protein S27a; CEP52; Chap1; epididymis luminal protein 112; epididymis secretory protein Li 50; hPLIC-2; HRIHFB2157; HUBCEP52; OTTHUMP00000200786; OTTHUMP00000201692; PLIC-2; polyubiquitin B; Polyubiquitin-B; Polyubiquitin-C; Ribosomal protein eS27-like; ribosomal protein S27-like; RPL40; RPS27A; RPS27L; Small ribosomal subunit protein eS27-like; UBA52; UBB; UBC; Ubiquilin 2; Ubiquitin A-52 residue ribosomal protein fusion product 1; ubiquitin and ribosomal protein S27a; ubiquitin C; ubiquitin carboxyl extension protein 52; Ubiquitin carboxyl extension protein 80; ubiquitin-52 amino acid fusion protein; ubiquitin-CEP52; ubiquitin-CEP80; Ubiquitin-ribosomal protein eL40 fusion protein; Ubiquitin-ribosomal protein eS31 fusion protein

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基因别名: CEP52; CEP80; HEL-S-50; HEL112; HMG20; HUBCEP52; L40; RPL40; RPS27A; RPS27L; S27A; UBA52; UBA80; UBB; UBC; UBCEP1; UBCEP2; UBCEP80

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UniProt ID: (Human) P62979, (Human) Q71UM5, (Human) P62987, (Human) P0CG47, (Human) P0CG48

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Entrez Gene ID: (Human) 6233, (Human) 51065, (Human) 7311, (Human) 7314, (Human) 7316

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功能
structural constituent of ribosome protein binding poly(A) RNA binding metal ion binding translation activator activity cysteine-type endopeptidase activator activity involved in apoptotic process protease binding ribosomal protein translational protein
参与通路
G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade nuclear-transcribed mRNA catabolic process, nonsense-mediated decay activation of MAPK activity protein polyubiquitination nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding stimulatory C-type lectin receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway glycogen biosynthetic process transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling rRNA processing translation translational initiation SRP-dependent cotranslational protein targeting to membrane DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest transforming growth factor beta receptor signaling pathway Notch signaling pathway I-kappaB kinase/NF-kappaB signaling JNK cascade fibroblast growth factor receptor signaling pathway regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death Wnt signaling pathway endosomal transport macroautophagy viral life cycle virion assembly viral transcription translesion synthesis negative regulation of transforming growth factor beta receptor signaling pathway anaphase-promoting complex-dependent catabolic process regulation of type I interferon production negative regulation of type I interferon production tumor necrosis factor-mediated signaling pathway nucleotide-excision repair, DNA incision ion transmembrane transport TRIF-dependent toll-like receptor signaling pathway interstrand cross-link repair NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway error-prone translesion synthesis DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability cellular protein metabolic process innate immune response positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia nucleotide-binding oligomerization domain containing signaling pathway global genome nucleotide-excision repair error-free translesion synthesis intracellular transport of virus negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of signal transduction by p53 class mediator ribosomal small subunit assembly DNA repair activation of cysteine-type endopeptidase activity involved in apoptotic process cellular response to DNA damage stimulus mitotic G1 DNA damage checkpoint intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator positive regulation of translation positive regulation of defense response to virus by host cellular protein modification process xenophagy positive regulation of protein ubiquitination mitochondrion transport along microtubule neuron projection morphogenesis regulation of mitochondrial membrane potential regulation of proteasomal protein catabolic process regulation of neuron death positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator positive regulation of protein monoubiquitination ubiquitin homeostasis
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