Invitrogen

Ubiquitin B Monoclonal Antibody (3C12)

查看其他5个Ubiquitin B抗体

抗体检测数据 (3)

Ubiquitin B Antibody in Western Blot (WB)

Ubiquitin B Antibody in Western Blot (WB)

图: 1 / 3

Ubiquitin B Antibody (MA5-17196) in WB

Western blot analysis of UBB using UBB monoclonal antibody (Product # MA5-17196) in NIH/3T3 (1) and HeLa (2) cell lysate.

Ubiquitin B Antibody in Western Blot (WB)

Ubiquitin B Antibody in Western Blot (WB)

Ubiquitin B Antibody in Flow Cytometry (Flow)

Product Image Gallery

Ubiquitin B Antibody in Western Blot (WB) — Western blot analysis of UBB using UBB monoclonal antibody (Product # MA5-17196) in NIH/3T3 (1) and HeLa (2) cell lysate.

Ubiquitin B Antibody in Western Blot (WB) — Western blot analysis of UBB using a UBB monoclonal antibody (Product # MA5-17196) against a human UBB (AA: 1-299) recombinant protein.

Ubiquitin B Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of HeLa cells using UBB monoclonal antibody (Product # MA5-17196) (green) and negative control (red).

产品信息

MA5-17196

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

-

流式细胞分析 (Flow)

1:200-1:400

-

酶联免疫吸附实验 (ELISA)

1:10,000

-

产品规格

种属反应

Human, Mouse

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3C12

抗原

Purified recombinant fragment of human UBB expressed in E. Coli.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein G

保存液

PBS

内含物

0.05% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2538667

产品详细信息

MA5-17196 targets UBB in FACS, indirect ELISA, and WB applications and shows reactivity with Human samples.

The MA5-17196 immunogen is purified recombinant fragment of human UBB expressed in E. Coli.

MA5-17196 detects UBB which has a predicted molecular weight of approximately 26kDa.

靶标信息

Ubiquitin is a highly conserved protein of about 8.5 kDa molecular weight, which has an ATP dependent role in the targeting of proteins for proteolytic degradation. To perform this function, the protein to be degraded is first covalently attached to the C terminus of ubiquitin, and the ubiquitinated complex is then recognized by a complex of degradative enzymes. Interestingly, ubiquitin also becomes covalently bonded to many types of pathological inclusions, which appear to be resistant to normal degradation. Therefore, ubiquitin antibodies are very useful for studies of these inclusions. For example, the neurofibrillary tangles and paired helical filaments diagnostic of Alzheimer's disease, Lewy bodies seen in Parkinson's disease, and Pick bodies found in Pick's disease are all heavily ubiquitinated and can be readily visualized with ubiquitin antibodies.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: epididymis secretory protein Li 50; HUBCEP52; MGC127041; polyubiquitin B; Polyubiquitin-B; RPL40

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基因别名: AL033289; HEL-S-50; Rps27a; Uba52; UBB; Ubb2; Ubc

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UniProt ID: (Human) P0CG47, (Mouse) P0CG49

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Entrez Gene ID: (Human) 7314, (Mouse) 22187

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protein binding

参与通路

male meiosis I female meiosis I male gonad development female gonad development ubiquitin homeostasis hypothalamus gonadotrophin-releasing hormone neuron development mitochondrion transport along microtubule neuron projection morphogenesis regulation of mitochondrial membrane potential adipose tissue development fat pad development regulation of proteasomal protein catabolic process seminiferous tubule development energy homeostasis regulation of neuron death positive regulation of intrinsic apoptotic signaling pathway by p53 class mediator G2/M transition of mitotic cell cycle negative regulation of transcription from RNA polymerase II promoter MAPK cascade activation of MAPK activity protein polyubiquitination nucleotide-excision repair, DNA damage recognition nucleotide-excision repair, DNA duplex unwinding stimulatory C-type lectin receptor signaling pathway MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway glycogen biosynthetic process transcription-coupled nucleotide-excision repair nucleotide-excision repair, preincision complex assembly nucleotide-excision repair, DNA incision, 5'-to lesion nucleotide-excision repair, DNA gap filling DNA damage response, signal transduction by p53 class mediator resulting in cell cycle arrest transforming growth factor beta receptor signaling pathway Notch signaling pathway I-kappaB kinase/NF-kappaB signaling JNK cascade fibroblast growth factor receptor signaling pathway regulation of tumor necrosis factor-mediated signaling pathway regulation of necrotic cell death Wnt signaling pathway endosomal transport macroautophagy viral life cycle virion assembly translesion synthesis negative regulation of transforming growth factor beta receptor signaling pathway anaphase-promoting complex-dependent catabolic process positive regulation of protein ubiquitination regulation of type I interferon production negative regulation of type I interferon production tumor necrosis factor-mediated signaling pathway nucleotide-excision repair, DNA incision ion transmembrane transport TRIF-dependent toll-like receptor signaling pathway interstrand cross-link repair NIK/NF-kappaB signaling Fc-epsilon receptor signaling pathway ERBB2 signaling pathway negative regulation of epidermal growth factor receptor signaling pathway error-prone translesion synthesis DNA damage response, detection of DNA damage protein ubiquitination involved in ubiquitin-dependent protein catabolic process positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of I-kappaB kinase/NF-kappaB signaling proteasome-mediated ubiquitin-dependent protein catabolic process regulation of mRNA stability cellular protein metabolic process innate immune response positive regulation of epidermal growth factor receptor signaling pathway positive regulation of transcription from RNA polymerase II promoter T cell receptor signaling pathway positive regulation of NF-kappaB transcription factor activity stress-activated MAPK cascade negative regulation of ubiquitin-protein ligase activity involved in mitotic cell cycle positive regulation of ubiquitin-protein ligase activity involved in regulation of mitotic cell cycle transition Wnt signaling pathway, planar cell polarity pathway regulation of transcription from RNA polymerase II promoter in response to hypoxia nucleotide-binding oligomerization domain containing signaling pathway global genome nucleotide-excision repair error-free translesion synthesis intracellular transport of virus negative regulation of canonical Wnt signaling pathway positive regulation of canonical Wnt signaling pathway regulation of signal transduction by p53 class mediator positive regulation of protein monoubiquitination