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Agrisera
V-ATPase, B/vacuolar H-ATPase subunit B Polyclonal Antibody
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V-ATPase, B/vacuolar H-ATPase subunit B Antibody (AS14-2775) in WB
Proteins were separated by SDS-PAGE and transferred to an Immobilon-P membrane (Millipore) using Trans-Blot SD Semi-Dry Transfer Cell (Bio-Rad) with transfer buffer (100 m M Tris, 192 m M Glycine, 0.02% (w/v) SDS and 5% (v/v) methanol). After treatment with 1% blocking agent, the membrane filter was incubated with the primary antibody (1:1,000) and then with horseradish peroxidase (HRP)-conjugated goat anti... View More
产品信息
AS14-2775
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide chosen from Arabidopsis thaliana V-ATPase subunit B, isoform B1, isoform B2, and isoform B3.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 100 µL of sterile water.
Specific Species Reactivity: Arabidopsis halleri, Nicotiana tabaccum, Thellungiella salsuginea, Vigna radiata
靶标信息
V ATPase B is the B subunit of the plant vacuolar type proton ATPase that forms part of the peripheral V1 catalytic sector responsible for ATP hydrolysis. Together with other V1 and V0 subunits, it drives proton pumping across vacuolar and endomembrane membranes to acidify lumens, generating the proton motive force that powers secondary transport of ions and metabolites. The B subunit helps organize the catalytic hexamer, interacts with regulatory proteins, and contributes to coupling between ATP turnover and proton translocation. V ATPase activity underpins pH homeostasis, vesicle trafficking, protein sorting, and stress responses, influencing processes such as stomatal function, nutrient storage, and growth. Expression of B subunits is developmentally and environmentally regulated, and perturbation alters vacuolar acidification, ion balance, and resilience to drought, salinity, and pathogens.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: AtVAB1; AtVAB2; AtVAB3; VAB1; VAB2; VAB3; VHA-B1; VHA-B2; VHA-B3
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