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Invitrogen
V-ATPase E1 Polyclonal Antibody
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图: 1 / 11
V-ATPase E1 Antibody (PA5-29899) in ICC/IF
Immunofluorescence analysis of V-ATPase E1 was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with anti-V-ATPase E1 Rabbit Polyclonal Antibody (Product # PA5-29899) at 1:200 dilution in 0.1% BSA, incubated at ... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
PA5-29899
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
已发表种属
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Recombinant protein encompassing a sequence within the center region of human ATP6V1E1. The exact sequence is proprietary.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
1.73 mg/mL
规格
173 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7, with 20% glycerol
内含物
0.025% ProClin 300
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recommended positive controls: A549, H1299, HCT116, mouse brain, rat brain.
Predicted reactivity: Mouse (99%), Rat (98%), Xenopus laevis (82%), Chicken (87%), Bovine (98%).
Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.
靶标信息
Subunit of the peripheral V1 complex of vacuolar ATPase essential for assembly or catalytic function. V-ATPase is responsible for acidifying a variety of intracellular compartments in eukaryotic cells.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: ATPase, H+ transporting lysosomal (vacuolar proton pump), 32 kDa; ATPase, H+ transporting, lysosomal 31kDa, V1 subunit E; ATPase, H+ transporting, V1 subunit E; H(+)-ATPase E-like protein; H+ ATPase subunit E; lysosomal 31kDa; p31; V-ATPase 31 kDa subunit; V-ATPase subunit E; V-ATPase subunit E 1; V-type proton ATPase subunit E 1; vacuolar H(+)-ATPase, E subunit; vacuolar H+ ATPase E1; Vacuolar proton pump subunit E 1; VATPase, H+ transporting, lysosomal V1 subunit E1
基因别名: 2410029D23Rik; ATP6E; ATP6E2; Atp6v1e; ATP6V1E1; D6Ertd385e; E2; P31; Vma4
UniProt ID: (Mouse) P50518, (Rat) Q6PCU2
Entrez Gene ID: (Mouse) 11973, (Rat) 297566
Disclaimer
Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
