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Agrisera
V-ATPase, a1/vacuolar H-ATPase subunit a isoform 1 Polyclonal Antibody
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V-ATPase, a1/vacuolar H-ATPase subunit a isoform 1 Antibody (AS14-2822) in WB
10 µg of microsomal membranes were isolated from 6-days-old etiolated (dark-grown) Arabidopsis thaliana seedlings with Extraction buffer (450 m M sucrose, 50 m M HEPES p H 7.5,5 m M Mg Cl 2,1 m M DTT, 1 x protease inhibitor). Proteins were denaturated in SDS sample buffer for 5 min at 95°C. Proteins were separated on a 10% SDS-PAGE and blotted 1 h to a nitrocellulose membrane using tank transfer. Blots were... View More
产品信息
AS14-2822
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit A.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
V-ATPase a1 (encoded by ATP6V0A1) is an integral membrane subunit of the vacuolar-type H+-ATPase (V-ATPase) that localizes predominantly to intracellular membranes, including endosomes and lysosomes, and is also enriched in neuronal compartments such as synaptic vesicles/clathrin-coated vesicles where it supports neurotransmitter loading. Structurally, V-ATPase is organized into a peripheral V1 ATP-hydrolytic sector and a membrane-embedded V0 proton-translocation sector; a1 is the V0 "a" isoform that provides a large N-terminal cytosolic domain important for V1-V0 assembly/regulation and a C-terminal multi-pass transmembrane region that interfaces with the proteolipid ring to enable proton movement across the membrane. Functionally, a1-containing V-ATPase complexes use ATP hydrolysis to pump protons into organelle lumens, generating acidity and electrochemical gradients required for protein sorting and degradation, receptor-mediated endocytosis, and vesicular trafficking, and in neurons specifically to establish the proton motive force that drives vesicular neurotransmitter uptake; consistent with this role, disease-associated ATP6V0A1 variants impair lysosomal acidification.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: T17D12.8; T17D12_8; VHA-A1
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