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Agrisera
V-ATPase, a3/vacuolar H-ATPase subunit a isoform 3 Polyclonal Antibody
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V-ATPase, a3/vacuolar H-ATPase subunit a isoform 3 Antibody (AS20-4369) in WB
30 µg of microsomal membranes were extracted freshly from 5-day-old Arabidopsis thaliana seedlings grown in liquid culture with a buffer containing 350 m M sucrose, 70 m M Tris-HCl p H 8,10% glycerol, 3 m M Na 2 EDTA, 0.15% BSA, 1.5% PVP-40,4 m M DTT, 1 x Roche complete™ Protease Inhibitor and denatured with 1 x LämmLi buffer at 50°C for 10 min. Proteins were separated on 7,5% SDS-PAGE and blotted 1 h to PV... View More
产品信息
AS20-4369
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated synthetic peptide derived from Arabidopsis thaliana V-ATPase subunit a3.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
V-ATPase a3 (encoded by TCIRG1) is a membrane-embedded "a" subunit of the V0 sector of the vacuolar-type H+ ATPase (V-ATPase), a rotary proton pump that acidifies intracellular compartments and, in specialized cells, the extracellular space adjacent to the plasma membrane. In osteoclasts, a3-containing V-ATPase is enriched on secretory lysosomes and is delivered to the ruffled border membrane, where it drives proton secretion to acidify the resorption lacuna required for bone demineralization and matrix degradation; in addition, a3 contributes to secretory lysosome trafficking by recruiting the small GTPase Rab7 to lysosomal membranes. Structurally, a3 is a large integral membrane protein forming part of the V0 proton-translocation machinery, with a substantial cytosolic N-terminal domain implicated in regulatory/trafficking interactions and a multi-pass C-terminal transmembrane domain that participates in the membrane channel interface with the proteolipid ring. Loss-of-function variants disrupt organelle/extracellular acidification and osteoclast polarity/ruffled border formation, consistent with the established association of TCIRG1 defects with infantile malignant osteopetrosis.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: a3; Atp6i; ATP6N1C; ATP6V0A3; OC-116kDa; OC116; OPTB1; Stv1; TIRC7; Vph1
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