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Invitrogen
V5 Tag Recombinant Rabbit Monoclonal Antibody (SY30-01)
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V5 Tag Antibody (MA5-32053) in ICC/IF
Immunofluorescent analysis of V5 Tag was performed using 70% confluent V5-H3-6XHis transfected HEK-293 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with V5 Tag Recombinant Rabbit Monoclonal Antibody (SY30-01) (Product # MA5-32053) at 5 µg/mL and Histone H3 Monoclonal Antibody (865R2) (Product # AHO1432) at 1:200 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then lab... View More
产品信息
MA5-32053
应用
建议稀释比
已发表文章
产品规格
种属反应
Tag
已发表种属
C. elegans,
Tag
宿主/亚型
Rabbit
/ IgG
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
克隆号
SY30-01
抗原
Synthetic peptide immune sequence is CGKPIPNPLLGLDST
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
TBS, pH 7.4, with 40% Glycerol, 0.05% BSA
内含物
0.05% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.
靶标信息
Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. The V5 epitope tag is derived from a small epitope (Pk) present on the P and V proteins of the paramyxovirus of simian virus (SV5). The V5 tag is usually used with all 14 amino acids (GKPIPNPLLGLDST), although it has also been used with a shorter 9 amino acid sequence (IPNPLLGLD).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
