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Invitrogen
VCAM-1 Monoclonal Antibody (1.4C3)
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VCAM-1 Antibody (MA5-11447) in ICC/IF
Immunofluorescent analysis of CD106 (green) showing staining in the cytoplasm of HUVEC cells treated with human TNF-alpha (right) compared to untreated HUVEC cells (left). Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with a CD106 monoclonal antibody (Product # MA5-11447) in 3% BSA-PBS at a dilution of 1:20 and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight... View More
产品信息
MA5-11447
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
C. elegans,
Human,
Mouse,
Rat
宿主/亚型
Mouse
/ IgG1, kappa
分类
Monoclonal
类型
Antibody
克隆号
1.4C3
抗原
Stimulated human umbilical vein endothelial cells (HUVEC)
偶联物
形式
Liquid
浓度
0.2 mg/mL
纯化类型
Protein A/G
保存液
PBS, pH 7.4, with 0.05% BSA
内含物
0.05% sodium azide
保存条件
4°C
运输条件
Ambient (domestic); Wet ice (international)
RRID
产品详细信息
MA5-11447 targets CD106 in IHC (P, F) and ICC/IF applications and shows reactivity with Human samples.
The MA5-11447 immunogen is stimulated human umbilical vein endothelial cells (HUVEC).
MA5-11447 has been successfully used in immunohistochemistry analysis of VCAM1 in mouse E16.5 frozen lung sections.
靶标信息
VCAM-1 is a cell surface sialoglycoprotein expressed by cytokine activated endothelium. The protein has a number of functions including the regulation of leukocyte migration, leukocyte-endothelial cell adhesion and signal transduction and may play a role in a number of inflammatory diseases.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: CD106; CD106 antigen; DKFZp779G2333; INCAM-100; INCAM100; MGC99561; sCD106; soluble CD106; soluble VCAM 1; sVCAM 1; V-CAM 1; Vascular cell adhesion protein 1
基因别名: CD106; INCAM-100; Vcam-1; VCAM1
UniProt ID: (Human) P19320
Entrez Gene ID: (Human) 7412, (Mouse) 22329
response to hypoxia
acute inflammatory response
chronic inflammatory response
cell adhesion
heterophilic cell-cell adhesion via plasma membrane cell adhesion molecules
leukocyte cell-cell adhesion
cell-matrix adhesion
aging
response to nutrient
amine metabolic process
response to zinc ion
response to ionizing radiation
viral process
membrane to membrane docking
B cell differentiation
extracellular matrix organization
response to lipopolysaccharide
response to nicotine
calcium-mediated signaling using intracellular calcium source
cellular response to vascular endothelial growth factor stimulus
positive regulation of T cell proliferation
response to ethanol
regulation of immune response
leukocyte tethering or rolling
oxidation-reduction process
cell chemotaxis
interferon-gamma-mediated signaling pathway
cellular response to tumor necrosis factor
heart development
single organismal cell-cell adhesion
embryonic placenta morphogenesis
chorio-allantoic fusion
visceral serous pericardium development
cellular response to glucose stimulus
