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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • VEGF-165 Antibodies

          Invitrogen

          VEGF-165 Polyclonal Antibody, PeproTech®

          1个已发表图片
          6 篇参考文献
          查看其他12个VEGF-165抗体

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          说明书
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          技术支持
          说明书
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          问题与答案
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          Cite VEGF-165 Polyclonal Antibody, PeproTech®

          • 抗体检测数据 (3)
          • 已发表图片 (1)
          VEGF-165 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          VEGF-165 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 4

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          VEGF-165 Antibody (500-P131-1MG) in WB

          Western Blot: To detect Murine VEGF 165 by Western Blot analysis VEGF-165 Polyclonal Antibody (Product # 500-P131-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine VEGF165 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          VEGF-165 Antibody in Western Blot (WB)
          VEGF-165 Antibody in Western Blot (WB)
          VEGF-165 Antibody in ELISA (ELISA)
          VEGF-165 Antibody in Western Blot (WB)

          产品信息

          500-P131-1MG

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          0.1-0.2 µg/mL
          查看3篇已发表文章 3篇已发表文章

          流式细胞分析 (Flow)

          -
          查看1篇已发表文章 1篇已发表文章

          酶联免疫吸附实验 (ELISA)

          0.5-2.0 µg/mL
          查看1篇已发表文章 1篇已发表文章

          中和实验 (Neu)

          -
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Mouse

          已发表种属

          Mouse, Rat

          宿主/亚型

          Rabbit

          分类

          Polyclonal

          类型

          Antibody

          抗原

          E.coli-derived Recombinant Murine VEGF 165

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Lyophilized

          浓度

          0.1-1.0 mg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS

          内含物

          no preservative

          保存条件

          -20°C

          运输条件

          Ambient

          RRID

          AB_2929389

          产品详细信息

          AA Sequence of recombinant protein: MAPTTEGEQK SHEVIKFMDV YQRSYCRPIE TLVDIFQEYP DEIEYIFKPS CVPLMRCAGC CNDEALECVP TSESNITMQI MRIKPHQSQH IGEMSFLQHS RCECRPKKDR TKPEKHCEPC SERRKHLFVQ DPQTCKCSCK NTDSRCKARQ LELNERTCRC DKPRR.

          Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine VEGF 165. Anti-Murine VEGF 165-specific antibody was purified by affinity chromatography employing an immobilized Murine VEGF 165 matrix.

          Sandwich ELISA: To detect Murine VEGF 165 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine VEGF 165 (500-P131Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine VEGF 165.

          Western Blot: To detect Murine VEGF 165 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine VEGF165 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

          靶标信息

          The vascular endothelial growth factor (VEGF) family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: H-VEGF-165; L-VEGF; M-VEGF-165; vascular endothelial growth factor 165b; vascular endothelial growth factor A; Vascular endothelial growth factor A, long form; Vascular permeability factor; VEGF165; VPF

          查看更多 收起

          基因别名: Vegf; Vegfa; Vpf

          查看更多 收起

          UniProt ID: (Mouse) Q00731

          查看更多 收起

          Entrez Gene ID: (Mouse) 22339

          查看更多 收起

          功能
          fibronectin binding receptor binding cytokine activity platelet-derived growth factor receptor binding vascular endothelial growth factor receptor binding protein binding growth factor activity heparin binding neuropilin binding chemoattractant activity identical protein binding protein homodimerization activity vascular endothelial growth factor receptor 1 binding vascular endothelial growth factor receptor 2 binding protein heterodimerization activity receptor agonist activity extracellular matrix binding growth factor
          参与通路
          negative regulation of transcription from RNA polymerase II promoter angiogenesis ovarian follicle development blood vessel development patterning of blood vessels response to hypoxia in utero embryonic development kidney development positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation vasculature development lymphangiogenesis blood vessel remodeling sprouting angiogenesis cell migration involved in sprouting angiogenesis positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation positive regulation of receptor internalization basophil chemotaxis heart morphogenesis outflow tract morphogenesis coronary vein morphogenesis regulation of transcription from RNA polymerase II promoter multicellular organism development nervous system development mesoderm development lactation cell proliferation positive regulation of cell proliferation regulation of cell shape positive regulation of signal transduction positive regulation of endothelial cell migration positive regulation of gene expression cell migration negative regulation of cell-cell adhesion cell differentiation monocyte differentiation macrophage differentiation lung development positive regulation of cell migration regulation of cGMP metabolic process epithelial cell differentiation positive regulation of vascular endothelial growth factor receptor signaling pathway post-embryonic camera-type eye development positive regulation of protein complex assembly positive regulation of protein autophosphorylation activation of protein kinase activity positive regulation of CREB transcription factor activity positive regulation of peptidyl-serine phosphorylation tube formation endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus lymph vessel morphogenesis positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway vascular endothelial growth factor signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway VEGF-activated neuropilin signaling pathway growth T-helper 1 type immune response eye photoreceptor cell development negative regulation of apoptotic process negative regulation of programmed cell death positive regulation of vascular permeability surfactant homeostasis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity negative regulation of neuron apoptotic process positive regulation of blood vessel endothelial cell migration endothelial cell migration regulation of endothelial cell differentiation positive regulation of angiogenesis negative regulation of bone resorption positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway mRNA stabilization lung alveolus development cell maturation blood vessel morphogenesis camera-type eye morphogenesis positive regulation of smooth muscle cell proliferation neuron development cardiac muscle fiber development branching morphogenesis of an epithelial tube positive regulation of axon extension involved in axon guidance artery morphogenesis positive regulation of epithelial cell proliferation positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of positive chemotaxis induction of positive chemotaxis positive regulation of cellular component movement positive regulation of cell division positive regulation of focal adhesion assembly positive regulation of protein kinase B signaling primitive erythrocyte differentiation cell chemotaxis lung vasculature development mammary gland alveolus development positive regulation of mast cell chemotaxis cardiac vascular smooth muscle cell development angiogenesis involved in coronary vascular morphogenesis coronary artery morphogenesis positive regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of ERK1 and ERK2 cascade cellular response to hypoxia dopaminergic neuron differentiation commissural neuron axon guidance positive regulation of protein kinase C signaling positive regulation of cell migration involved in sprouting angiogenesis positive regulation of branching involved in ureteric bud morphogenesis regulation of retinal ganglion cell axon guidance positive regulation of peptidyl-tyrosine autophosphorylation positive regulation of p38MAPK cascade negative regulation of neuron death positive regulation of lymphangiogenesis regulation of hematopoietic progenitor cell differentiation positive regulation of histone deacetylase activity positive regulation of retinal ganglion cell axon guidance positive regulation of protein localization to early endosome positive regulation of protein kinase D signaling positive regulation of receptor activity negative regulation of extrinsic apoptotic signaling pathway
          It has to be done as per old AB suggested Products section.
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