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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • VEGF Antibodies

          Invitrogen

          VEGF Polyclonal Antibody

          2个已发表图片
          5 篇参考文献
          查看其他51个VEGF抗体

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          说明书
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          Cite VEGF Polyclonal Antibody

          • 抗体检测数据 (2)
          • 已发表图片 (2)
          VEGF Antibody in Western Blot (WB)
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          VEGF Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 4

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          VEGF Antibody (PA1-16948) in WB

          Western blot was performed using Anti-VEGF Polyclonal Antibody (Product # PA1-16948) and 20 and 40 kDa bands corresponding to VEGF were observed in recombinant VEGF protein. Recombinant VEGF protein (250 ng) was electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0321BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23002) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1 µg/mL) and detected by chemiluminescence with Goat anti-Rabb... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          查看产品

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          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)
          VEGF Antibody in Western Blot (WB)

          产品信息

          PA1-16948

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1 µg/mL
          查看2篇已发表文章 2篇已发表文章

          免疫组化 (IHC)

          -
          查看1篇已发表文章 1篇已发表文章

          免疫组化(石蜡) (IHC (P))

          -
          查看1篇已发表文章 1篇已发表文章

          酶联免疫吸附实验 (ELISA)

          1:500
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Bovine, Dog, Chicken, Horse, Guinea pig, Human, Mouse, Rat

          已发表种属

          Guinea pig, Human, Mouse

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          A synthetic peptide made to an internal region of the human VEGFA protein sequence (between residues 180-232).

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS

          内含物

          0.09% sodium azide

          保存条件

          4°C, do not freeze

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2288261

          产品详细信息

          In Western blot, a band is seen at ~42 kDa with a chimera transfected lysate representing the homodimer VEGFA.

          Another faint band is seen at ~21 kDa, representing the monodomer form of VEGFA.

          This antibody is not recommended for IHC applications.Suggested positive control: antigen standard for VEGFA (transient overexpression lysate).

          靶标信息

          VEGF (vascular endothelial growth factor) which is a 45 kDa homodimeric, disulfide-linked glycoprotein involved in angiogenesis which promotes tumor progression and metastasis. VEGF has a variety of effects on vascular endothelium, including the ability to promote endothelial cell viability, mitogenesis, chemotaxis, and vascular permeability. The VEGF family currently includes VEGF-A, VEGF-B, VEGF-C, VEGF-D, VEGF-E, and PIGF. VEGF and its receptor system have been shown to be the fundamental regulators in the cell signaling of angiogenesis. Most tumors have the absolute requirement of angiogenesis, and VEGF has been described as the most potent angiogenic cytokine linked to this process. To date 5 different isoforms of VEGF have been described. These isoforms are generated as the result of alternative splicing from a single VEGF gene. These various isoforms have been shown to bind to two tyrosine-kinase receptors flt-1 (VEGFR-1) and flk-1/KDR (VEGFR-2), which have been found to be expressed almost exclusively on endothelial cells. VEGF and its high-affinity binding receptors, the tyrosine kinases FLK1 and FLT1, are thought to be important for the development of embryonic vasculature. Studies have shown that an alternately spliced form of FLT1 produces a soluble protein, termed sFLT1, which binds vascular endothelial growth factor with high affinity, playing an inhibitory role in angiogenesis. Elevated levels of VEGF is linked to POEMS syndrome (Polyneuropathy, Organomegaly, Endocrinopathy, Monoclonal gammopathy, Skin changes) also known as Crow-Fukase syndrome which affects multiple organs in the body.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: L-VEGF; MGC70609; RP1-261G23.1; VAS; vascular endothelial growth factor; vascular endothelial growth factor (VEGF) precursor; vascular endothelial growth factor 164; vascular endothelial growth factor 165b; vascular endothelial growth factor 188; Vascular endothelial growth factor A; Vascular endothelial growth factor A, long form; vascular endothelial growth factor A121; vascular endothelial growth factor A165; Vascular permeability factor; Vasculotropin; VEGF; VEGF-A; VEGF1; VEGFMGC70609; VPF; VPF ant

          查看更多 收起

          基因别名: eVEGF120; eVEGF164; MVCD1; VEGF; VEGF-A; VEGF164; VEGFA; VPF

          查看更多 收起

          UniProt ID: (Bovine) P15691, (Human) P15692, (Chicken) P67964, (Horse) Q9GKR0, (Dog) Q9MYV3, (Rat) P16612, (Mouse) Q00731

          查看更多 收起

          Entrez Gene ID: (Bovine) 281572, (Human) 7422, (Chicken) 395909, (Horse) 100033839, (Dog) 403802, (Rat) 83785, (Guinea pig) 100713483, (Mouse) 22339

          查看更多 收起

          功能
          cytokine activity growth factor activity heparin binding protein homodimerization activity fibronectin binding platelet-derived growth factor receptor binding vascular endothelial growth factor receptor binding protein binding chemoattractant activity identical protein binding vascular endothelial growth factor receptor 1 binding vascular endothelial growth factor receptor 2 binding protein heterodimerization activity receptor agonist activity extracellular matrix binding receptor binding neuropilin binding growth factor
          参与通路
          positive regulation of protein phosphorylation positive regulation of endothelial cell proliferation positive regulation of endothelial cell migration positive regulation of protein complex assembly tube formation positive regulation of angiogenesis positive regulation of peptidyl-tyrosine phosphorylation positive chemotaxis positive regulation of cell division positive regulation of focal adhesion assembly cellular response to hypoxia motor neuron migration negative regulation of transcription from RNA polymerase II promoter angiogenesis ovarian follicle development patterning of blood vessels vasculogenesis response to hypoxia in utero embryonic development kidney development cell migration involved in sprouting angiogenesis positive regulation of neuroblast proliferation positive regulation of mesenchymal cell proliferation positive regulation of receptor internalization platelet degranulation positive regulation of leukocyte migration heart morphogenesis outflow tract morphogenesis coronary vein morphogenesis nervous system development mesoderm development lactation positive regulation of cell proliferation regulation of cell shape positive regulation of gene expression monocyte differentiation macrophage differentiation lung development positive regulation of cell migration regulation of cGMP metabolic process epithelial cell differentiation positive regulation of vascular endothelial growth factor receptor signaling pathway post-embryonic camera-type eye development positive regulation of protein autophosphorylation activation of protein kinase activity positive regulation of CREB transcription factor activity positive regulation of peptidyl-serine phosphorylation endothelial cell chemotaxis cellular response to vascular endothelial growth factor stimulus lymph vessel morphogenesis positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway positive regulation of cell proliferation by VEGF-activated platelet derived growth factor receptor signaling pathway VEGF-activated neuropilin signaling pathway growth eye photoreceptor cell development positive regulation of tyrosine phosphorylation of Stat3 protein negative regulation of apoptotic process surfactant homeostasis negative regulation of cysteine-type endopeptidase activity involved in apoptotic process positive regulation of MAP kinase activity positive regulation of blood vessel endothelial cell migration positive regulation of cell adhesion positive regulation of transcription from RNA polymerase II promoter vascular endothelial growth factor receptor signaling pathway cell maturation camera-type eye morphogenesis cardiac muscle fiber development branching morphogenesis of an epithelial tube positive regulation of axon extension involved in axon guidance artery morphogenesis positive regulation of epithelial cell proliferation positive regulation of positive chemotaxis induction of positive chemotaxis positive regulation of cellular component movement primitive erythrocyte differentiation mammary gland alveolus development cardiac vascular smooth muscle cell development coronary artery morphogenesis regulation of transcription from RNA polymerase II promoter in response to hypoxia positive regulation of transcription from RNA polymerase II promoter in response to hypoxia dopaminergic neuron differentiation commissural neuron axon guidance positive regulation of protein kinase C signaling positive regulation of cell migration involved in sprouting angiogenesis positive regulation of branching involved in ureteric bud morphogenesis regulation of retinal ganglion cell axon guidance positive regulation of peptidyl-tyrosine autophosphorylation positive regulation of p38MAPK cascade positive regulation of histone deacetylase activity positive regulation of retinal ganglion cell axon guidance positive regulation of protein kinase D signaling blood vessel maturation sprouting angiogenesis epicardial cell to mesenchymal cell transition vascular endothelial growth factor signaling pathway positive regulation of vascular permeability positive regulation of mast cell chemotaxis blood vessel development vasculature development lymphangiogenesis blood vessel remodeling basophil chemotaxis regulation of transcription from RNA polymerase II promoter female pregnancy aging cell proliferation response to cold positive regulation of signal transduction cell migration negative regulation of cell-cell adhesion cell differentiation hyaluronan metabolic process response to estradiol response to progesterone response to vitamin A cellular response to drug wound healing T-helper 1 type immune response negative regulation of programmed cell death negative regulation of neuron apoptotic process endothelial cell migration cellular response to fibroblast growth factor stimulus regulation of endothelial cell differentiation negative regulation of bone resorption mRNA stabilization lung alveolus development blood vessel morphogenesis positive regulation of smooth muscle cell proliferation neuron development response to folic acid positive regulation of protein kinase B signaling cell chemotaxis lung vasculature development angiogenesis involved in coronary vascular morphogenesis positive regulation of ERK1 and ERK2 cascade response to oxygen levels response to dexamethasone cellular response to dexamethasone stimulus negative regulation of neuron death positive regulation of lymphangiogenesis regulation of hematopoietic progenitor cell differentiation positive regulation of protein localization to early endosome negative regulation of extrinsic apoptotic signaling pathway multicellular organism development positive regulation of receptor activity
          It has to be done as per old AB suggested Products section.
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